The First Anti-Snakebite and Hepatoprotective Characterization of a Trypsin Kunitz-like Inhibitor (EcTI) from the Plant Enterolobium contortisiliquum: a Case of Two Soul Mates Meeting

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Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorPorto University-
Autor(es): dc.creatorCosta, Caroline R. C.-
Autor(es): dc.creatorBelchor, Mariana N.-
Autor(es): dc.creatorRoggero, Airam-
Autor(es): dc.creatorMoraes, Laila L.-
Autor(es): dc.creatorSamelo, Ricardo-
Autor(es): dc.creatorAnnunciato, Isabelly-
Autor(es): dc.creatorBonturi, Camila R.-
Autor(es): dc.creatorOliva, Maria L. V.-
Autor(es): dc.creatorSousa, Sergio F.-
Autor(es): dc.creatorde Oliveira, Marcos A.-
Autor(es): dc.creatorToyama, Marcos H.-
Data de aceite: dc.date.accessioned2025-08-21T19:18:09Z-
Data de disponibilização: dc.date.available2025-08-21T19:18:09Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ph16040632-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249914-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249914-
Descrição: dc.descriptionSnake venom serine protease (SVSP) interferes with the regulation and control of important biological reactions in homeostasis and can be classified as an activator of the fibrinolytic system and platelet aggregation. Our group has recently isolated a new serine protease from Crotalus durissus terrificus total venom (Cdtsp-2). This protein exhibits edematogenic capacity and myotoxic activity. A Kunitz-like EcTI inhibitor protein with a molecular mass of 20 kDa was isolated from Enterolobium contortisiliquum and showed high trypsin inhibition. Thus, the objective of this work is to verify the possible inhibition of the pharmacological activities of Cdtsp-2 by the Kutinz-type inhibitor EcTI. To isolate Cdtsp-2 from total C. d. terrificus venom, we used three-step chromatographic HPLC. Using the mice paw edema model, we observed an edematogenic effect, myotoxicity and hepatotoxicity caused by Cdtsp-2. In vitro and in vivo experiments showed that the alterations in hemostasis caused by Cdtsp-2 are crucial for the development of marked hepatotoxicity and that EcTI significantly inhibits the enzymatic and pharmacological activities of Cdtsp-2. Kunitz-like inhibitor may be a viable alternative for the development of ancillary treatments against the biological activities of venoms.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCenter of Natural and Human Sciences Federal University of ABC (UFABC), SP-
Descrição: dc.descriptionUniversity of São Paulo State (UNESP), Biosciences Institute of Paulista Coast Campus (IB/CLP), SP-
Descrição: dc.descriptionNational Institute of Pharmacology (INFAR) Federal University of São Paulo (UNIFESP), SP-
Descrição: dc.descriptionUnit of Applied Biomolecular Sciences (UCIBIO) REQUIMTE-BioSIM-Medicine Faculty Porto University-
Descrição: dc.descriptionUniversity of São Paulo State (UNESP), Biosciences Institute of Paulista Coast Campus (IB/CLP), SP-
Descrição: dc.descriptionFAPESP: 2003/10516-2-
Descrição: dc.descriptionFAPESP: 2017/20291-0-
Descrição: dc.descriptionCNPq: 309271/2022-3-
Descrição: dc.descriptionFAPESP: p2017/19942-7-
Idioma: dc.languageen-
Relação: dc.relationPharmaceuticals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCdtsp-2-
Palavras-chave: dc.subjectCrotalus durissus terrificus-
Palavras-chave: dc.subjectKunitz-type inhibitor-
Palavras-chave: dc.subjectserine protease-
Título: dc.titleThe First Anti-Snakebite and Hepatoprotective Characterization of a Trypsin Kunitz-like Inhibitor (EcTI) from the Plant Enterolobium contortisiliquum: a Case of Two Soul Mates Meeting-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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