MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDe Freitas, Jéssica Henrique-
Autor(es): dc.creatorBragato, Jaqueline Poleto-
Autor(es): dc.creatorRebech, Gabriela Torres-
Autor(es): dc.creatorCosta, Sidnei Ferro-
Autor(es): dc.creatorDos Santos, Marilene Oliveira-
Autor(es): dc.creatorSoares, Matheus Fujimura-
Autor(es): dc.creatorEugênio, Flávia de Rezende-
Autor(es): dc.creatorDos Santos, Paulo Sérgio Patto-
Autor(es): dc.creatorDe Lima, Valéria Marçal Felix-
Data de aceite: dc.date.accessioned2025-08-21T16:52:16Z-
Data de disponibilização: dc.date.available2025-08-21T16:52:16Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fgene.2023.1106496-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249899-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249899-
Descrição: dc.descriptionCanine Visceral leishmaniasis (CanL) poses a severe public health threat in several countries. Disease progression depends on the degree of immune response suppression. MicroRNAs (miRs) modulate mRNA translation into proteins and regulate various cellular functions and pathways associated with immune responses. MiR-21 and miR-148a can alter the parasite load and M1 macrophages are the principal cells in dogs’ leishmanicidal activity. A previous study found increased miR-21 and miR-148a in splenic leukocytes (SL) of dogs with CanL using microarray analysis and in silico analysis identified PTEN pathway targets. PTEN is involved in the immune regulation of macrophages. We measured PTEN and the production of reactive oxygen species (ROS) and nitric oxide (NO) before and after transfection SLs of dogs with CanL with mimic and inhibition of miR-21 and miR-148a. PTEN levels increased, NO and ROS decreased in SLs from dogs with CanL. Inhibition of miRNA-21 resulted in PTEN increase; in contrast, PTEN decreased after miR-148a inhibition. Nitrite (NO2) levels increased after transfection with miR-21 inhibitor but were decreased with miR-148a inhibitor. The increase in miR-21 promoted a reduction in ROS and NO levels, but miR-148a inhibition increased NO and reduced ROS. These findings suggest that miR-21 and miR-148a can participate in immune response in CanL, affecting PTEN, NO, and ROS levels.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Animal Clinic School of Veterinary Medicine Surgery and Reproduction São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/17261-5 2021/07283-4-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Genetics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectLeishmania infantum-
Palavras-chave: dc.subjectmicroRNA-
Palavras-chave: dc.subjectnitrite-
Palavras-chave: dc.subjectNO-
Palavras-chave: dc.subjectpten-
Palavras-chave: dc.subjectROS-
Palavras-chave: dc.subjectsplenic leukocytes-
Título: dc.titleMicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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