Development, characterization and in vitro cytotoxicity of kaempferol-loaded nanostructured lipid carriers in glioblastoma multiforme cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorNicoleti, Luisa Ribeiro-
Autor(es): dc.creatorDi Filippo, Leonardo Delello-
Autor(es): dc.creatorDuarte, Jonatas Lobato-
Autor(es): dc.creatorLuiz, Marcela Tavares-
Autor(es): dc.creatorSábio, Rafael Miguel-
Autor(es): dc.creatorChorilli, Marlus-
Data de aceite: dc.date.accessioned2025-08-21T21:21:41Z-
Data de disponibilização: dc.date.available2025-08-21T21:21:41Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2023.113309-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249846-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249846-
Descrição: dc.descriptionGlioblastoma multiforme is the most common and most aggressive human brain cancer. GBM treatment is still a challenge because many drugs are not able to cross the blood-brain barrier, in addition to the increasing resistance to currently available chemotherapy. New therapeutic alternatives are emerging, and, in this context, we highlight kaempferol, a flavonoid with remarkable anti-tumor activity but with limited bioavailability due to its strong lipophilic property. A promising tool to improve the biopharmaceutical properties of molecules such as kaempferol is the use of drug-delivery nanosystems, such as nanostructured lipid carriers (NLC), which can facilitate the dispersion and delivery of highly lipophilic molecules. The present work aimed at the development and characterization of kaempferol-loaded NLC (K-NLC) and the evaluation of its biological properties using in vitro models. The K-NLC showed an average size of 120 nm, zeta potential of − 21 mV, and polydispersity index of 0.099. The K-NLC presented high kaempferol encapsulation efficiency (93%), a drug loading of 3.58%, and a sustained kaempferol release profile for up to 48 h. In addition to presenting a 7-fold increase in kaempferol cytotoxicity, its encapsulation in NLC promoted a cellular uptake of 75%, which corroborates with increased cytotoxicity in U-87MG cells, as observed. Together, these data reinforce the promising antineoplastic properties of kaempferol in addition to the key role of NLC as a platform for the efficient delivery of lipophilic drugs to neoplastic cells, which improved their uptake and therapeutic efficacy in glioblastoma multiforme cells.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University “Júlio de Mesquita Filho” School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionSão Paulo State University “Júlio de Mesquita Filho” School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionFAPESP: 19/17913–5-
Descrição: dc.descriptionFAPESP: 2019/25125–7-
Descrição: dc.descriptionFAPESP: 2020/12622–0-
Idioma: dc.languageen-
Relação: dc.relationColloids and Surfaces B: Biointerfaces-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrain cancer-
Palavras-chave: dc.subjectDrug delivery-
Palavras-chave: dc.subjectFlavonoids-
Palavras-chave: dc.subjectLipid nanoparticles-
Título: dc.titleDevelopment, characterization and in vitro cytotoxicity of kaempferol-loaded nanostructured lipid carriers in glioblastoma multiforme cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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