Venlafaxine increases aromatization, reduces apical V-ATPase in clear cells and induces increased number of mast cells and smooth muscle cells death in rat cauda epididymis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSchool of Medicine-
Autor(es): dc.creatorda Silva, André Acácio Souza-
Autor(es): dc.creatorde Santi, Fabiane-
Autor(es): dc.creatorHinton, Barry T.-
Autor(es): dc.creatorCerri, Paulo Sérgio-
Autor(es): dc.creatorSasso-Cerri, Estela-
Data de aceite: dc.date.accessioned2025-08-21T20:45:12Z-
Data de disponibilização: dc.date.available2025-08-21T20:45:12Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-02-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2022.121329-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249577-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249577-
Descrição: dc.descriptionDepressive disorders (DD) have affected millions of people worldwide. Venlafaxine, antidepressant of the class of serotonin and norepinephrine reuptake inhibitors, has been prescribed for the treatment of DD. In rat testes, venlafaxine induces testosterone (T) aromatization and increases estrogen levels. Aromatase is a key enzyme for the formation of estrogen in the epididymis, an essential organ for male fertility. We investigated the impact of serotonergic/noradrenergic venlafaxine effect on the epididymal cauda region, focusing on aromatase, V-ATPase and EGF epithelial immunoexpression, smooth muscle (SM) integrity and mast cells number (MCN). Male rats were distributed into control (CG; n = 10) and venlafaxine (VFG, n = 10) groups. VFG received 30 mg/kg b.w. of venlafaxine for 35 days. The epididymal cauda was processed for light and transmission electron microscopy (TEM). The expression of connexin 43 (Cx43) and estrogen alpha (Esr1), adrenergic (Adra1a) and serotonergic (Htr1b) receptors were analyzed. Clear cells (CCs) area, SM thickness, viable spermatozoa (VS) and MCN were evaluated. Apoptosis was confirmed by TUNEL and TEM. The following immunoreactions were performed: T, aromatase, T/aromatase co-localization, V-ATPase, EGF, Cx43 and PCNA. The increased Adra1a and reduced Htr1b expressions confirmed the noradrenergic and serotonergic venlafaxine effects, respectively, corroborating the increased MCN, apoptosis and atrophy of SM. In VFG, the epithelial EGF increased, explaining Cx43 overexpression and basal cells mitotic activity. T aromatization and Esr1 downregulation indicate high estrogen levels, explaining CCs hypertrophy and changes in the V-ATPase localization, corroborating VS reduction. Thus, in addition to serotonergic/noradrenergic effects, T/estrogen imbalance, induced by venlafaxine, impairs epididymal structure and function.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Morphology Genetics Orthodontics and Pediatric Dentistry-
Descrição: dc.descriptionFederal University of São Paulo Department of Morphology and Genetics-
Descrição: dc.descriptionUniversity of Virginia School of Medicine Department of Cell Biology-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Department of Morphology Genetics Orthodontics and Pediatric Dentistry-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/19829-6-
Descrição: dc.descriptionFAPESP: 2018/25353-7-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntidepressant-
Palavras-chave: dc.subjectApoptosis-
Palavras-chave: dc.subjectClear cells-
Palavras-chave: dc.subjectImmunofluorescence-
Palavras-chave: dc.subjectMast cells-
Palavras-chave: dc.subjectSNRI-
Título: dc.titleVenlafaxine increases aromatization, reduces apical V-ATPase in clear cells and induces increased number of mast cells and smooth muscle cells death in rat cauda epididymis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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