Participation of fibroblast growth factor-1 and interleukin-10 in connective tissue repair following subcutaneous implantation of bioceramic materials in rats

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDelfino, Mateus Machado-
Autor(es): dc.creatorJampani, José Leandro de Abreu-
Autor(es): dc.creatorLopes, Camila Soares-
Autor(es): dc.creatorGuerreiro-Tanomaru, Juliane Maria-
Autor(es): dc.creatorTanomaru-Filho, Mário-
Autor(es): dc.creatorSasso-Cerri, Estela-
Autor(es): dc.creatorCerri, Paulo Sérgio-
Data de aceite: dc.date.accessioned2025-08-21T16:05:29Z-
Data de disponibilização: dc.date.available2025-08-21T16:05:29Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.13867-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249400-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249400-
Descrição: dc.descriptionAim: To evaluate whether the bioceramic materials Bio-C Pulpo (Bio-C, Angelus) and mineral trioxide aggregate (MTA) Repair HP (MTA-HP, Angelus) induce fibroblast proliferation and release of interleukin-10 (IL-10), an anti-inflammatory cytokine, stimulating connective tissue remodelling. The tissue response of Bio-C and MTA-HP was compared with the White MTA (WMTA; Angelus) since studies have demonstrated that WMTA induces tissue repair. Methodology: Bio-C, MTA-HP and WMTA were inserted into polyethylene tubes and implanted in the subcutaneous tissue of Holtzman rats for 7, 15, 30 and 60 days. As a control group (CG), empty tubes were implanted subcutaneously. The number of fibroblasts (FB), Ki-67-, fibroblast growth factor-1- (FGF-1) and IL-10-immunolabelled cells and collagen content in the capsules was obtained. The data were subjected to two-way anova followed by Tukey's test (p ≤.05). Results: At 7 days, significant differences in the number of FB were not detected amongst Bio-C, MTA-HP and WMTA groups (p ˃.05). The capsules of all groups exhibited a significant increase in the number of FB and content of collagen over time. From 7 to 60 days, a significant reduction in the number of FGF-1- and Ki-67-immunolabelled cells was seen in the capsules of all specimens. In all periods, no significant difference in the number of FGF-1-immunolabelled cells was detected between Bio-C and CG specimens. At 60 days, significant differences in the immunoexpression of FGF-1 were not observed amongst the groups. At 7 and 15 days, the highest immunoexpression for Ki-67 was present in Bio-C specimens whilst, after 30 and 60 days, no significant difference was observed amongst the bioceramic materials. At 7 days, few IL-10 immunolabelled cells were present in the capsules of all specimens whereas, at 60 days, a significant increase in the IL-10-immunostaining was present in all groups. At 60 days, the Bio-C, MTA-HP and WMTA groups showed a greater number of IL-10-immunolabelled cells than in the CG specimens (p <.0001). Conclusions: Bio-C, MTA-HP and WMTA stimulate fibroblast proliferation, leading to the formation of collagen-rich capsules. FGF-1 and IL-10 may mediate the remodelling of capsules around Bio-C, MTA-HP and WMTA bioceramic materials.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2018/16848-2-
Descrição: dc.descriptionFAPESP: 2019/17739-5-
Formato: dc.format385-401-
Idioma: dc.languageen-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbioceramic materials-
Palavras-chave: dc.subjectcell proliferation-
Palavras-chave: dc.subjectcollagen-
Palavras-chave: dc.subjectimmunohistochemistry-
Palavras-chave: dc.subjectinterleukin-10-
Palavras-chave: dc.subjectKi-67-
Título: dc.titleParticipation of fibroblast growth factor-1 and interleukin-10 in connective tissue repair following subcutaneous implantation of bioceramic materials in rats-
Tipo de arquivo: dc.typelivro digital-
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