Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

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Autor(es): dc.contributorUniversidade Federal da Bahia (UFBA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Pelotas-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.creatorBarral, Thiago Doria-
Autor(es): dc.creatorKalil, Mauricio Alcantara-
Autor(es): dc.creatorMariutti, Ricardo Barros-
Autor(es): dc.creatorArni, Raghuvir Krishnaswamy-
Autor(es): dc.creatorGismene, Carolina-
Autor(es): dc.creatorSousa, Fernanda Severo-
Autor(es): dc.creatorCollares, Tiago-
Autor(es): dc.creatorSeixas, Fabiana Kommling-
Autor(es): dc.creatorBorsuk, Sibele-
Autor(es): dc.creatorEstrela-Lima, Alessandra-
Autor(es): dc.creatorAzevedo, Vasco-
Autor(es): dc.creatorMeyer, Roberto-
Autor(es): dc.creatorPortela, Ricardo Wagner-
Data de aceite: dc.date.accessioned2025-08-21T20:02:22Z-
Data de disponibilização: dc.date.available2025-08-21T20:02:22Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00253-022-12279-1-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249381-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249381-
Descrição: dc.descriptionAbstract: Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein was constructed by combining Corynebacterium pseudotuberculosis PLD and CP40 proteins with maltose-binding protein (MBP) as an intrinsic adjuvant. The antigenicity, allergenic potential, prediction of B epitopes, binding to MHC receptors, and docking on the Toll-Like 2 receptor were evaluated in silico. MBP:PLD:CP40 was expressed and purified. 40 BALB/c were divided into four groups (G1 – control, G2 – Saponin, G3 – MBP:PLD:CP40, and G4 – rPLD + rCP40). Total IgG, IgG1, and IgG2a were quantified, and the expressions of cytokines after splenocyte in vitro stimulation were assessed. Mice were challenged 42 days after the first immunization. The in silico analysis showed that MBP:PLD:CP40 has immunogenic potential, does not have allergic properties, and can dock on the TRL2 receptor. MBP:PLD:CP40 stimulated the production of IgG1 antibodies in a fivefold proportion to IgG2a, and TNF and IL-17 were significantly expressed in response to the antigenic stimuli. When rPLD and rCP40 were used together for immunization, they could induce IFN-γ and IL-12, but with no detectable antibody production. The G3 and G4 groups presented a survival of 57.14% and 42.86%, respectively, while the G1 and G2 mice were all dead 15 days after the challenge. MBP:PLD:CP40 partially protected the mice against C. pseudotuberculosis infection and can be considered a potential new CLA immunogen. Key points: • The fusion protein induced more IgG1 than IgG2a antibodies; • The fusion protein also induced the expression of the TNF and IL-17 cytokines; • Mice inoculated with MBP:PLD:CP40 presented a 57.14% survival.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLaboratory of Immunology and Molecular Biology Universidade Federal da Bahia, Avenida Reitor Miguel Calmon s/n, Bahia State-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation Universidade Estadual Paulista, São Paulo State-
Descrição: dc.descriptionCenter for Technological Development Universidade Federal de Pelotas, Rio Grande do Sul State-
Descrição: dc.descriptionLaboratory of Veterinary Pathology School of Veterinary Medicine and Zootechnics Universidade Federal da Bahia, Bahia State-
Descrição: dc.descriptionLaboratory of Molecular and Cellular Genetics Universidade Federal de Minas Gerais, Minas Gerais State-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation Universidade Estadual Paulista, São Paulo State-
Descrição: dc.descriptionCAPES: PhD Fellowship-
Descrição: dc.descriptionCNPq: Research Fellowship-
Descrição: dc.descriptionCNPq: Techogical Development Fellowship 313350/2019-1-
Formato: dc.format8035-8051-
Idioma: dc.languageen-
Relação: dc.relationApplied Microbiology and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCaseous lymphadenitis-
Palavras-chave: dc.subjectImmunoinformatics-
Palavras-chave: dc.subjectRecombinant proteins-
Palavras-chave: dc.subjectVaccine development-
Título: dc.titleImmunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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