Proteome profiling of methyl jasmonate elicitation of Maytenus ilicifolia in vitro roots reveals insights into sesquiterpene pyridine alkaloids

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de Ribeirão Preto (UNAERP)-
Autor(es): dc.contributorUniversidad de La Frontera (UFRO)-
Autor(es): dc.creatorSantos, Vânia A. F. F. M. dos-
Autor(es): dc.creatorCoppede, Juliana da Silva-
Autor(es): dc.creatorDias, Nathalia Batista-
Autor(es): dc.creatorPereira, Ana Maria Soares-
Autor(es): dc.creatorPalma, Mario Sergio-
Autor(es): dc.creatorFurlan, Maysa-
Data de aceite: dc.date.accessioned2025-08-21T19:15:44Z-
Data de disponibilização: dc.date.available2025-08-21T19:15:44Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11240-022-02371-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249338-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249338-
Descrição: dc.descriptionMaytenus ilicifolia (Celastraceae) is an important medicinal plant widely used in Brazil and a rich source of sesquiterpene pyridine alkaloids (SPAs) with relevant biological activities. They exhibit great chemical diversity and high structural complexity, and show accumulation in very low concentrations. Thus, to determine whether in vitro cultured adventitious roots of M. ilicifolia could provide alternative sources for the production of these compounds, the accumulation of SPAs ilicifoliunine A and aquifoliunine E-I, as well as the time of production and the content of these alkaloids were evaluated using methyl jasmonate (MeJA) as an elicitor. The results showed the accumulation ilicifoliunine A and aquifoliunine E-I and an increase in the contents of these alkaloids when elicited with MeJA at 7 and 28 days, respectively. To characterize and identify the proteins present in the enzymatic system and contribute to the understanding of the metabolic pathways involved in the biosynthesis of SPAs, was carried out in vitro proteomic profiling of adventitious roots elicited and non-elicited with MeJA. The shotgun proteomic analyses led to the identification of important proteins involved in the biosynthetic pathway of SPAs, including germacrene A synthase, β-eudesmol synthase, and L-aspartate oxidase. Additionally, many proteins that participate in the biosynthesis of other alkaloids (e.g., tropane, pyrrolidine, terpenoid indole, and isoquinoline) and phenylpropanoids have been identified. In conclusion, the study report for the first time, proteins involved in the biosynthetic pathway of SPAs by using the adventitious roots in vitro system of M. ilicifolia and a shotgun proteomic approach.-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartamento de Biotecnologia Vegetal Universidade de Ribeirão Preto (UNAERP)-
Descrição: dc.descriptionScientific and Technological Bioresource Nucleus BIOREN-UFRO Universidad de La Frontera (UFRO)-
Descrição: dc.descriptionInstituto de Biociências Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Biociências Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista (UNESP)-
Formato: dc.format551-563-
Idioma: dc.languageen-
Relação: dc.relationPlant Cell, Tissue and Organ Culture-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCelastraceae-
Palavras-chave: dc.subjectIn vitro adventitious roots-
Palavras-chave: dc.subjectMaytenus ilicifolia-
Palavras-chave: dc.subjectMethyl jasmonate-
Palavras-chave: dc.subjectSesquiterpene pyridine alkaloids-
Palavras-chave: dc.subjectShotgun proteomic-
Título: dc.titleProteome profiling of methyl jasmonate elicitation of Maytenus ilicifolia in vitro roots reveals insights into sesquiterpene pyridine alkaloids-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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