Rubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVale do Itajaí University (UNIVALI)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorCECAV and Department of Genetics and Biotechnology-
Autor(es): dc.creatorDe Quadros, Ana Paula Oliveira-
Autor(es): dc.creatorOshiiwa, Bruna-
Autor(es): dc.creatorPetreanu, Marcel-
Autor(es): dc.creatorNiero, Rivaldo-
Autor(es): dc.creatorRosa, Paulo Cesar Pires-
Autor(es): dc.creatorSawaya, Alexandra Christine Helena Frankland-
Autor(es): dc.creatorMantovani, Mario Sergio-
Autor(es): dc.creatorO'Neill De Mascarenhas Gaivão, Isabel-
Autor(es): dc.creatorMaistro, Edson Luis-
Data de aceite: dc.date.accessioned2025-08-21T21:20:56Z-
Data de disponibilização: dc.date.available2025-08-21T21:20:56Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2022.105485-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249331-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249331-
Descrição: dc.descriptionRubus rosifolius, popularly known as “red mulberry”, is a common medicinal plant in southern Brazil and is used as an antidiarrheal, analgesic, antimicrobial and antihypertensive, and to treat stomach diseases. The aim of this study was to analyze the R. rosifolius stem extract (RrSE) for possible in vitro cytotoxic and genotoxic effects, using the comet assay and the micronucleus test to assess genotoxicity, and flow cytometry to assess the impact on the cell cycle and apoptosis in HepG2/C3A cells, in addition to evaluating the expression of genes linked to the induction of DNA damage, cell cycle, apoptosis and metabolism of xenobiotics. The MTT assay observed no cytotoxic effects at concentrations between 0.01 and 100 μg/mL of the extract. However, genotoxic effects occurred in treatments with the extract from a 1 μg/mL concentration. Flow cytometry analysis revealed a significant increase in cells in the G2/M phase after treatment with 10 μg/mL, a decrease in cells in the G0/G1 phase in the treatment with 100 μg/mL, and a significant increase in total apoptotic cells. In the gene expression analysis, an increase in the CYP1A2 xenobiotics metabolizing gene expression was observed. Despite the promising pharmacological effects of R. rosifolius, the results revealed that the RrSE has genotoxic effect and induces apoptosis in HepG2/C3A cells, indicating danger in using this plant extract by humans.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSão Paulo State University UNESP Biosciences Institute Post-Graduate Program on General and Applied Biology, SP-
Descrição: dc.descriptionSão Paulo State University UNESP Faculty of Philosophy and Sciences Speech and Hearing Therapy Department, SP-
Descrição: dc.descriptionVale do Itajaí University (UNIVALI) Department of Biological Sciences, SC-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences University of Campinas, Campinas-
Descrição: dc.descriptionLondrina State University UEL Department of General Biology, PR-
Descrição: dc.descriptionUniversity of Trás-os-Montes and Alto Douro CECAV and Department of Genetics and Biotechnology-
Descrição: dc.descriptionSão Paulo State University UNESP Biosciences Institute Post-Graduate Program on General and Applied Biology, SP-
Descrição: dc.descriptionSão Paulo State University UNESP Faculty of Philosophy and Sciences Speech and Hearing Therapy Department, SP-
Descrição: dc.descriptionFAPESP: 2017/24149-4-
Descrição: dc.descriptionCNPq: 303604/2021-2-
Idioma: dc.languageen-
Relação: dc.relationToxicology in Vitro-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApoptosis analysis-
Palavras-chave: dc.subjectCell cycle analysis-
Palavras-chave: dc.subjectComet assay-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectMicronucleus test-
Palavras-chave: dc.subjectRubus rosifolius toxicity-
Título: dc.titleRubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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