Phenylalanine ammonia lyase: new insights from Piperaceae species

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFEI University Center-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Araújo Morandim-Giannetti, Andreia-
Autor(es): dc.creatorFelippe, Lidiane Gaspareto-
Autor(es): dc.creatorde Freitas Formenton Macedo dos Santos, Vânia Aparecida-
Autor(es): dc.creatorKato, Massuo Jorge-
Autor(es): dc.creatorFurlan, Maysa-
Data de aceite: dc.date.accessioned2025-08-21T20:03:31Z-
Data de disponibilização: dc.date.available2025-08-21T20:03:31Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.26850/1678-4618EQJ.V47.2SI.2022.P67-82-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249186-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249186-
Descrição: dc.descriptionThe enzyme PAL (phenylalanine ammonia lyase) mediates the key entry point to the general phenylpropanoid pathway, which is involved in the lignification process and in the formation of a myriad of secondary compounds in plants that show a variety of biological activities. Soluble fractions containing PAL extracted from Piper and Peperomia species had the optimal catalytic activity analyzed by statistical design model. This analysis revealed that the best conversion of L-phenylalanine to trans-cinnamic acid was pH 9.3 and 58 °C after 25 h, corroborating interesting thermal stability. Additionally, the pre-purification of PAL using ammonium sulfate precipitation (25-55%) increased its specific activity, approximately 133% in P. aduncum and more than 900% in P. crassinervium. The content of lignin was higher for P. tuberculatum (25.71%), while only a small amount of lignin was observed in Peperomia blanda (11.95%). It is interesting to note that Peperomia plants are succulent and without significant amounts of lignin. However, the phenylpropanoid biosynthetic pathway is apparently addressed to produce predominantly tetrahydrofuran lignans with biological interest.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFEI University Center Department of Chemical Engeneering-
Descrição: dc.descriptionPaulista State University Institute of Chemistry-
Descrição: dc.descriptionUniversity of São Paulo Institute of Chemistry-
Descrição: dc.descriptionPaulista State University Institute of Chemistry-
Descrição: dc.descriptionFAPESP: 2013/07600-3-
Descrição: dc.descriptionCNPq: 2014/465637-0-
Descrição: dc.descriptionFAPESP: 2014/50926-0-
Formato: dc.format67-82-
Idioma: dc.languageen-
Relação: dc.relationEcletica Quimica-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectPeperomia-
Palavras-chave: dc.subjectphenylalanine ammonia lyase-
Palavras-chave: dc.subjectphenylpropanoid derivatives-
Palavras-chave: dc.subjectPiper-
Palavras-chave: dc.subjectThermal stability-
Título: dc.titlePhenylalanine ammonia lyase: new insights from Piperaceae species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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