Efflux Pumps and Multidrug-Resistance in Pyricularia oryzae Triticum Lineage

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIDR—Paraná/IAPAR-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.contributorNIAB-
Autor(es): dc.creatorVicentini, Samara Nunes Campos-
Autor(es): dc.creatorMoreira, Silvino Intra-
Autor(es): dc.creatorda Silva, Abimael Gomes-
Autor(es): dc.creatorde Oliveira, Tamiris Yoshie Kiyama-
Autor(es): dc.creatorSilva, Tatiane Carla-
Autor(es): dc.creatorAssis Junior, Fabio Gomes-
Autor(es): dc.creatorKrug, Loane Dantas-
Autor(es): dc.creatorde Paiva Custódio, Adriano Augusto-
Autor(es): dc.creatorLeite Júnior, Rui Pereira-
Autor(es): dc.creatorTeodoro, Paulo Eduardo-
Autor(es): dc.creatorFraaije, Bart-
Autor(es): dc.creatorCeresini, Paulo Cezar-
Data de aceite: dc.date.accessioned2025-08-21T19:40:52Z-
Data de disponibilização: dc.date.available2025-08-21T19:40:52Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/agronomy12092068-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249177-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249177-
Descrição: dc.descriptionWidespread resistance to QoIs, DMI and SDHIs fungicides has been reported for Brazilian populations of the wheat blast pathogen Pyricularia oryzae Triticum lineage (PoTl). A pre-existing resistance mechanism not associated with target site mutations has been indicated for resistance to DMIs and SDHIs, with strong indication that PoTl has multidrugresistance (MDR). Therefore, the main objective of this study was to test the hypothesis that resistance to DMI and SDHI fungicides detected in PoTl was due to efflux pump mediated MDR mechanism(s) by characterizing the sensitivity to antifungal efflux pump substrates. Four antifungal substrates were tested: tolnaftate (TOL), cycloheximide (CHX), rhodamine 6G (RH6G) and triphenyltin chloride (TPCL). TPCL and RH6G were considered the most relevant indicators for enhanced MDR activity. Among the 16 PoTl isolates tested, 9 were insensitive to TPCL, 1 to TOL, 16 to RH6G and 1 to CHX. The PoTl isolates were grouped into four distinct multidrug resistance phenotypes (MDRPs) based on resistance to combinations of fungicides and antifungal efflux pump substrates. Insensitivity to TPCL, RH6G and or TOL correlated well with DMI insensitivity, but MDR was not associated with SDHI resistance. The identification of multiple MDRP phenotypes associated with DMI resistance in our study warrants further research aimed at revealing the exact mechanisms of multidrug resistance in the wheat blast pathogen, including efflux pumps overexpression via transcriptomic analyses of differentially expressed genes; identification and discovery of mutations associated with changes in promoter regions or transcription factors of efflux transporters associated with multidrug resistance.-
Descrição: dc.descriptionNewton Fund-
Descrição: dc.descriptionDepartment of Crop Protection Agricultural Engineering and Soil (Departamento de Fitossanidade Engenharia Rural e Solos) Sao Paulo State University UNESP, SP-
Descrição: dc.descriptionParaná Rural Development Institute IDR—Paraná/IAPAR, PR-
Descrição: dc.descriptionFederal University of Mato Grosso do Sul UFMS, Campus CPSC, MS-
Descrição: dc.descriptionNational Institute of Agricultural Botany NIAB-
Descrição: dc.descriptionDepartment of Crop Protection Agricultural Engineering and Soil (Departamento de Fitossanidade Engenharia Rural e Solos) Sao Paulo State University UNESP, SP-
Descrição: dc.descriptionNewton Fund: BB/S018867/2-
Idioma: dc.languageen-
Relação: dc.relationAgronomy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDMI-
Palavras-chave: dc.subjectMDR-
Palavras-chave: dc.subjectQoI-
Palavras-chave: dc.subjectSDHI-
Palavras-chave: dc.subjectwheat blast-
Título: dc.titleEfflux Pumps and Multidrug-Resistance in Pyricularia oryzae Triticum Lineage-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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