Maquiberry Cystatins: Recombinant Expression, Characterization, and Use to Protect Tooth Dentin and Enamel

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorCatholic University of Maule (UCM)-
Autor(es): dc.creatorde Souza, Eduardo Pereira-
Autor(es): dc.creatorFerro, Milene-
Autor(es): dc.creatorPelá, Vinicius Taioqui-
Autor(es): dc.creatorFernanda-Carlos, Thais-
Autor(es): dc.creatorBorges, Cecília Guimarães Giannico-
Autor(es): dc.creatorTaira, Even Akemi-
Autor(es): dc.creatorVentura, Talita Mendes Oliveira-
Autor(es): dc.creatorArencibia, Ariel Domingo-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorHenrique-Silva, Flávio-
Data de aceite: dc.date.accessioned2025-08-21T20:33:00Z-
Data de disponibilização: dc.date.available2025-08-21T20:33:00Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biomedicines11051360-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249096-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249096-
Descrição: dc.descriptionPhytocystatins are proteinaceous competitive inhibitors of cysteine peptidases involved in physiological and defensive roles in plants. Their application as potential therapeutics for human disorders has been suggested, and the hunt for novel cystatin variants in different plants, such as maqui (Aristotelia chilensis), is pertinent. Being an understudied species, the biotechnological potential of maqui proteins is little understood. In the present study, we constructed a transcriptome of maqui plantlets using next-generation sequencing, in which we found six cystatin sequences. Five of them were cloned and recombinantly expressed. Inhibition assays were performed against papain and human cathepsins B and L. Maquicystatins can inhibit the proteases in nanomolar order, except MaquiCPIs 4 and 5, which inhibit cathepsin B in micromolar order. This suggests maquicystatins’ potential use for treating human diseases. In addition, since we previously demonstrated the efficacy of a sugarcane-derived cystatin to protect dental enamel, we tested the ability of MaquiCPI-3 to protect both dentin and enamel. Both were protected by this protein (by One-way ANOVA and Tukey’s Multiple Comparisons Test, p < 0.05), suggesting its potential usage in dental products.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos (UFSCar), SP-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo (USP), SP-
Descrição: dc.descriptionCenter of Biotechnology in Natural Resources Faculty of Agrarian and Forestry Sciences Catholic University of Maule (UCM)-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: 2019/26070-1-
Descrição: dc.descriptionFAPESP: 2021/11152-2-
Descrição: dc.descriptionFAPESP: 2022/13268-0-
Idioma: dc.languageen-
Relação: dc.relationBiomedicines-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAristotelia chilensis-
Palavras-chave: dc.subjectcathepsin-
Palavras-chave: dc.subjectcysteine protease-
Palavras-chave: dc.subjectcysteine protease inhibitor-
Palavras-chave: dc.subjectdental erosion-
Palavras-chave: dc.subjectdentistry-
Palavras-chave: dc.subjectphytocystatin-
Título: dc.titleMaquiberry Cystatins: Recombinant Expression, Characterization, and Use to Protect Tooth Dentin and Enamel-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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