A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity

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Autor(es): dc.contributorFederal University of Parana-
Autor(es): dc.contributorInstituto Carlos Chagas-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBrasil University-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.contributorVeterinarian-
Autor(es): dc.contributorFIOCRUZ-
Autor(es): dc.contributorUniversity São Paulo-
Autor(es): dc.contributorUniversity of Passo Fundo-
Autor(es): dc.contributorFederal University of Santa Maria-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorNord University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Ceará-
Autor(es): dc.contributorUrutai Campus-
Autor(es): dc.contributorFederal University of Tocantins-
Autor(es): dc.contributorFederal University of Mato Grosso do Sul-
Autor(es): dc.contributorNational Institute for Quality Control in Health-
Autor(es): dc.creatorBastos, Thais Sibioni Berti-
Autor(es): dc.creatorde Paula, André Guilherme Portela-
Autor(es): dc.creatordos Santos Luz, Rebeca Bosso-
Autor(es): dc.creatorGarnique, Anali M. B.-
Autor(es): dc.creatorBelo, Marco A. A.-
Autor(es): dc.creatorEto, Silas Fernandes-
Autor(es): dc.creatorFernandes, Dayanne Carla-
Autor(es): dc.creatorFerraris, Fausto Klabund-
Autor(es): dc.creatorde Pontes, Leticia Gomes-
Autor(es): dc.creatorFrança, Tábata Takahashi-
Autor(es): dc.creatorBarcellos, Leonardo José Gil-
Autor(es): dc.creatorVeras, Flavio P.-
Autor(es): dc.creatorBermejo, Pamela-
Autor(es): dc.creatorGuidelli, Giovanna-
Autor(es): dc.creatorManeira, Carla-
Autor(es): dc.creatorda Silveira Bezerra de Mello, Fellipe-
Autor(es): dc.creatorTeixeira, Gleidson-
Autor(es): dc.creatorPereira, Gonçalo Amarante Guimarães-
Autor(es): dc.creatorFernandes, Bianca H. Ventura-
Autor(es): dc.creatorSanches, Paulo R. S.-
Autor(es): dc.creatorBraz, Helyson Lucas Bezerra-
Autor(es): dc.creatorJorge, Roberta Jeane Bezerra-
Autor(es): dc.creatorMalafaia, Guilherme-
Autor(es): dc.creatorCilli, Eduardo M.-
Autor(es): dc.creatorOlivier, Danilo da Silva-
Autor(es): dc.creatordo Amaral, Marcos Serrou-
Autor(es): dc.creatorMedeiros, Renata J.-
Autor(es): dc.creatorCondino-Neto, Antonio-
Autor(es): dc.creatorCarvalho, Luciani R.-
Autor(es): dc.creatorMachado-Santelli, Glaucia M.-
Autor(es): dc.creatorCharlie-Silva, Ives-
Autor(es): dc.creatorGalindo-Villegas, Jorge-
Autor(es): dc.creatorBraga, Tárcio Teodoro-
Data de aceite: dc.date.accessioned2025-08-21T16:28:41Z-
Data de disponibilização: dc.date.available2025-08-21T16:28:41Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-023-29588-8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249082-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249082-
Descrição: dc.descriptionDespite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for the development of new technologies and therapies for the best clinical management of patients. Since the manipulation of the whole virus requires a structure with an adequate level of biosafety, the development of alternative technologies, such as the synthesis of peptides from viral proteins, is a possible solution to circumvent this problem. In addition, the use and validation of animal models is of extreme importance to screen new drugs and to compress the organism's response to the disease. Peptides derived from recombinant S protein from SARS-CoV-2 were synthesized and validated by in silico, in vitro and in vivo methodologies. Macrophages and neutrophils were challenged with the peptides and the production of inflammatory mediators and activation profile were evaluated. These peptides were also inoculated into the swim bladder of transgenic zebrafish larvae at 6 days post fertilization (dpf) to mimic the inflammatory process triggered by the virus, which was evaluated by confocal microscopy. In addition, toxicity and oxidative stress assays were also developed. In silico and molecular dynamics assays revealed that the peptides bind to the ACE2 receptor stably and interact with receptors and adhesion molecules, such as MHC and TCR, from humans and zebrafish. Macrophages stimulated with one of the peptides showed increased production of NO, TNF-α and CXCL2. Inoculation of the peptides in zebrafish larvae triggered an inflammatory process marked by macrophage recruitment and increased mortality, as well as histopathological changes, similarly to what is observed in individuals with COVID-19. The use of peptides is a valuable alternative for the study of host immune response in the context of COVID-19. The use of zebrafish as an animal model also proved to be appropriate and effective in evaluating the inflammatory process, comparable to humans.-
Descrição: dc.descriptionDepartment of Pathology Federal University of Parana-
Descrição: dc.descriptionGraduate Program in Biosciences and Biotechnology Instituto Carlos Chagas-
Descrição: dc.descriptionDepartment of Cell Biology Institute of Biomedical Sciences University of São Paulo-
Descrição: dc.descriptionBrasil University-
Descrição: dc.descriptionCenter of Excellence in New Target Discovery (CENTD) Special Laboratory Butantan Institute-
Descrição: dc.descriptionCenter of Innovation and Development Laboratory of Development and Innovation Butantan Institute-
Descrição: dc.descriptionVeterinarian-
Descrição: dc.descriptionDepartment of Pharmacology and Toxicology Oswaldo Cruz Foundation FIOCRUZ-
Descrição: dc.descriptionLaboratory of Human Immunology Department Immunology Institute Biomedical Sciences University São Paulo-
Descrição: dc.descriptionLaboratory of Fish Physiology Graduate Program of Bioexperimentation University of Passo Fundo-
Descrição: dc.descriptionGraduate Program of Pharmacology Federal University of Santa Maria-
Descrição: dc.descriptionCenter of Research in Inflammatory Diseases Ribeirão Preto Medical School University of Sao Paulo-
Descrição: dc.descriptionDepartment of Pharmacology Ribeirao Preto Medical School University of São Paulo-
Descrição: dc.descriptionLaboratório de Genômica e bioEnergia (LGE) Institute of Biology - Unicamp-
Descrição: dc.descriptionDepartment of Genomics Faculty of Biosciences and Aquaculture Nord University-
Descrição: dc.descriptionLaboratório de Controle Genético e Sanitário Diretoria Técnica de Apoio ao Ensino e Pesquisa Faculdade de Medicina da Universidade de São Paulo-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista, SP-
Descrição: dc.descriptionDepartment of Physiology and Pharmacology School of Medicine Federal University of Ceará, CE-
Descrição: dc.descriptionBiological Research Laboratory Goiano Federal Institute Urutai Campus, GO-
Descrição: dc.descriptionIntegrated Sciences Center Federal University of Tocantins, TO-
Descrição: dc.descriptionInstitute of Physics Federal University of Mato Grosso do Sul, MS-
Descrição: dc.descriptionLaboratory of Physiology INCQS/Fiocruz Zebrafish Facility Department of Pharmacology and Toxicology National Institute for Quality Control in Health-
Descrição: dc.descriptionLaboratory of Cellular and Molecular Biology Department of Cell and Developmental Biology Institute of Biomedical Science University of Sao Paulo University of São Paulo-
Descrição: dc.descriptionDepartment of Pharmacology University of São Paulo-ICB/USP-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista, SP-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleA novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity-
Tipo de arquivo: dc.typelivro digital-
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