Effect of proteins isolated from Brazilian snakes on enterovirus A71 replication cycle: An approach against hand, foot and mouth disease

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBrazilian Centre for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorUniversity of Leeds-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorShimizu, Jacqueline Farinha-
Autor(es): dc.creatorFeferbaum-Leite, Shiraz-
Autor(es): dc.creatorSantos, Igor Andrade-
Autor(es): dc.creatorMartins, Daniel Oliveira Silva-
Autor(es): dc.creatorKingston, Natalie J.-
Autor(es): dc.creatorShegdar, Mona-
Autor(es): dc.creatorZothner, Carsten-
Autor(es): dc.creatorSampaio, Suely Vilela-
Autor(es): dc.creatorHarris, Mark-
Autor(es): dc.creatorStonehouse, Nicola J.-
Autor(es): dc.creatorJardim, Ana Carolina Gomes-
Data de aceite: dc.date.accessioned2025-08-21T20:56:21Z-
Data de disponibilização: dc.date.available2025-08-21T20:56:21Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-06-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2023.124519-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249068-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249068-
Descrição: dc.descriptionEnterovirus A71 (EVA71) belongs to the Picornaviridae family and is the main etiological agent of hand, foot, and mouth disease (HFMD). There is no approved antiviral against EVA71, and therefore the search for novel anti-EVA71 therapeutics is essential. In this context, the antiviral activity of proteins isolated from snake venoms has been reported against a range of viruses. Here, the proteins CM10 and CM14 isolated from Bothrops moojeni, and Crotamin and PLA2CB isolated from Crotalus durissus terrificus were investigated for their antiviral activity against EVA71 infection. CM14 and Crotamin possessed a selective index (SI) of 170.8 and 120.4, respectively, while CM10 and PLA2CB had an SI of 67.4 and 12.5, respectively. CM14 inhibited all steps of viral replication (protective effect: 76 %; virucidal: 99 %; and post-entry: 99 %). Similarly, Crotamin inhibited up to 99 % of three steps. In contrast, CM10 and PLA2CB impaired one or two steps of EVA71 replication, respectively. Further dose-response assays using increasing titres of EVA71 were performed and CM14 and Crotamin retained functionality with high concentrations of EVA71 (up to 1000 TCID50). These data demonstrate that proteins isolated from snake venom are potent inhibitors of EVA71 and could be used as scaffolds for future development of novel antivirals.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionMedical Research Council-
Descrição: dc.descriptionLaboratory of Antiviral Research Institute of Biomedical Science — ICBIM Federal University of Uberlândia — UFU, MG-
Descrição: dc.descriptionInstitute of Biosciences Language and Exact Science — IBILCE São Paulo State University — UNESP, SP-
Descrição: dc.descriptionBrazilian Biosciences National Laboratory (LNBio) Brazilian Centre for Research in Energy and Materials (CNPEM), SP-
Descrição: dc.descriptionSchool of Molecular and Cellular Biology Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology University of Leeds-
Descrição: dc.descriptionDepartment of Clinical Analyses Toxicology and Food Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo — USP, SP-
Descrição: dc.descriptionInstitute of Biosciences Language and Exact Science — IBILCE São Paulo State University — UNESP, SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 142495/2020-4-
Descrição: dc.descriptionCAPES: 88881.506794/2020-01-
Descrição: dc.descriptionCAPES: 88887.703841/2022-00-
Descrição: dc.descriptionFAPEMIG: APQ-01487-22-
Descrição: dc.descriptionFAPEMIG: APQ-04686-22-
Descrição: dc.descriptionMedical Research Council: MR/P022626/1-
Descrição: dc.descriptionMedical Research Council: MR/S001026/1-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntiviral-
Palavras-chave: dc.subjectBrazilian snakes-
Palavras-chave: dc.subjectEnterovirus A71-
Palavras-chave: dc.subjectEVA71-
Palavras-chave: dc.subjectToxins-
Título: dc.titleEffect of proteins isolated from Brazilian snakes on enterovirus A71 replication cycle: An approach against hand, foot and mouth disease-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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