Lung Inflammation Induced by Inactivated SARS-CoV-2 in C57BL/6 Female Mice Is Controlled by Intranasal Instillation of Vitamin D

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorFernandes de Souza, William Danilo-
Autor(es): dc.creatorZorzella-Pezavento, Sofia Fernanda Gonçalves-
Autor(es): dc.creatorAyupe, Marina Caçador-
Autor(es): dc.creatorSalgado, Caio Loureiro-
Autor(es): dc.creatorOliveira, Bernardo de Castro-
Autor(es): dc.creatorMoreira, Francielly-
Autor(es): dc.creatorda Silva, Guilherme William-
Autor(es): dc.creatorMuraro, Stefanie Primon-
Autor(es): dc.creatorde Souza, Gabriela Fabiano-
Autor(es): dc.creatorProença-Módena, José Luiz-
Autor(es): dc.creatorAraujo Junior, Joao Pessoa-
Autor(es): dc.creatorFonseca, Denise Morais da-
Autor(es): dc.creatorSartori, Alexandrina-
Data de aceite: dc.date.accessioned2025-08-21T21:48:56Z-
Data de disponibilização: dc.date.available2025-08-21T21:48:56Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cells12071092-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249065-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249065-
Descrição: dc.descriptionThe COVID-19 pandemic was triggered by the coronavirus SARS-CoV-2, whose peak occurred in the years 2020 and 2021. The main target of this virus is the lung, and the infection is associated with an accentuated inflammatory process involving mainly the innate arm of the immune system. Here, we described the induction of a pulmonary inflammatory process triggered by the intranasal (IN) instillation of UV-inactivated SARS-CoV-2 in C57BL/6 female mice, and then the evaluation of the ability of vitamin D (VitD) to control this process. The assays used to estimate the severity of lung involvement included the total and differential number of cells in the bronchoalveolar lavage fluid (BALF), histopathological analysis, quantification of T cell subsets, and inflammatory mediators by RT-PCR, cytokine quantification in lung homogenates, and flow cytometric analysis of cells recovered from lung parenchyma. The IN instillation of inactivated SARS-CoV-2 triggered a pulmonary inflammatory process, consisting of various cell types and mediators, resembling the typical inflammation found in transgenic mice infected with SARS-CoV-2. This inflammatory process was significantly decreased by the IN delivery of VitD, but not by its IP administration, suggesting that this hormone could have a therapeutic potential in COVID-19 if locally applied. To our knowledge, the local delivery of VitD to downmodulate lung inflammation in COVID-19 is an original proposition.-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Mucosal Immunology Department of Immunology Institute of Biomedical Sciences University of São Paulo (USP), SP-
Descrição: dc.descriptionLaboratory of Emerging Viruses Department of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationCells-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectlung-
Palavras-chave: dc.subjectmice-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectvitamin D-
Título: dc.titleLung Inflammation Induced by Inactivated SARS-CoV-2 in C57BL/6 Female Mice Is Controlled by Intranasal Instillation of Vitamin D-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.