Molecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorMichigan State University-
Autor(es): dc.contributorInstituto Adolfo Lutz (IAL)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMcGill University-
Autor(es): dc.creatorGonçalves, Juliano Leonel-
Autor(es): dc.creatorLee, Sarah H. I.-
Autor(es): dc.creatorCamargo, Carlos H.-
Autor(es): dc.creatorZanella, Rosemeire Cobo-
Autor(es): dc.creatorSilva, Nathália C. C.-
Autor(es): dc.creatorRall, Vera. L. M.-
Autor(es): dc.creatorCue, Roger I.-
Autor(es): dc.creatordos Santos, Marcos V.-
Data de aceite: dc.date.accessioned2025-08-21T23:44:49Z-
Data de disponibilização: dc.date.available2025-08-21T23:44:49Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-023-00918-2-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249055-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249055-
Descrição: dc.descriptionThe study aimed to evaluate the genetic diversity of Staphylococcus aureus causing subclinical mastitis (SM) isolated from dairy cows and to assess the effect of the infection status (transient vs. persistent) on the milk and component yield. A total of six dairy farms in São Paulo state were used for the selection of cows with SM caused by S. aureus. S. aureus strains (n = 56) obtained from three biweekly aseptic mammary quarter milk samplings (n = 1140 from 95 cows) were subjected to MALDI-TOF MS analysis for species confirmation and further PFGE analysis. Intramammary infections (IMI) caused by S. aureus were categorized as transient (T: when only one out of 3 milk samplings had positive isolation of any pulsotype) or persistent (P: when two (P2) or three (P3) milk samplings had positive isolation of identical pulsotype over the consecutive episodes of SM. The SmaI macrorestriction fragment profiles of 56 S. aureus isolates showed a dominant S. aureus clonal pattern (PFGE type A; n = 50; 89.3%) within and among the herds. The SM-causing S. aureus represented a reduction of quarter milk yield of 26.2% in transient and 54.8% in persistent cases as well as a reduction of total solid yield of 38.1% and 49.4%, respectively, when compared with the healthy control quarters. Overall, the greater chance of S. aureus to be persistent is when a dominant clonal pattern is present in the herd which consequently may be associated with the cause of accentuated milk loss.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Animal Nutrition and Production School of Veterinary Medicine and Animal Sciences University of Sao Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University-
Descrição: dc.descriptionDepartment of Food Engineering Faculty of Animal Science and Food Engineering University of São Paulo, São Paulo-
Descrição: dc.descriptionCentro de Bacteriologia Instituto Adolfo Lutz (IAL), SP-
Descrição: dc.descriptionDepartment of Food Science and Nutrition Faculty of Food Engineering University of Campinas, São Paulo-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University, São Paulo-
Descrição: dc.descriptionDepartment of Animal Science McGill University, Macdonald Campus-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University, São Paulo-
Descrição: dc.descriptionFAPESP: BEPE 2015/04570-1)-
Descrição: dc.descriptionFAPESP: project funding (2014/17411-6)-
Descrição: dc.descriptionFAPESP: The authors ackledge FAPESP for the scholarship (2013/23613-8-
Formato: dc.format1181-1189-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChronic infection-
Palavras-chave: dc.subjectGenetic diversity-
Palavras-chave: dc.subjectMilk reduction-
Palavras-chave: dc.subjectPFGE-
Palavras-chave: dc.subjectStaphylococci-
Título: dc.titleMolecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.