Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidad Privada Norbert Wiener-
Autor(es): dc.contributorAlmirante Paulo Moreira Institute of Studies of the Sea-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.creatorSánchez-Moguel, Ignacio-
Autor(es): dc.creatorCosta-Silva, Tales A.-
Autor(es): dc.creatorPillaca-Pullo, Omar S.-
Autor(es): dc.creatorFlores-Santos, Juan Carlos-
Autor(es): dc.creatorFreire, Rominne Karla Barros-
Autor(es): dc.creatorCarretero, Gustavo-
Autor(es): dc.creatorda Luz Bueno, Júlia-
Autor(es): dc.creatorCamacho-Córdova, David I.-
Autor(es): dc.creatorSantos, João H.P.M.-
Autor(es): dc.creatorSette, Lara Durães-
Autor(es): dc.creatorPessoa-Jr, Adalberto-
Data de aceite: dc.date.accessioned2025-08-21T18:04:38Z-
Data de disponibilização: dc.date.available2025-08-21T18:04:38Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2023.03.003-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/249053-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/249053-
Descrição: dc.descriptionMicroorganisms from extreme environments, such as the Antarctic ecosystems, have a great potential to produce enzymes with novel characteristics. Within this context, L-asparaginase (ASNase) obtained from yeast species has been poorly studied. In this study, yeasts isolated from samples collected at Admiralty Bay (King George Island, Antarctica) were tested to produce ASNase. From an initial screening of 40 strains, belonging to 13 different species, Leucosporidium scottii L115 produced an ASNase activity (LsASNase activity: 6.24 U g-1 of dry cell weight) with the lowest glutaminase activity. The LsASNase was purified 227-fold, with a specific activity of 137.01 U mg-1 at 37 °C, without glutaminase activity. Moreover, the maximum enzyme activity was observed at pH 7.5 and at a temperature of 55 °C. The enzyme is a multimer of 462 kDa, presenting a single band of 53 kDa molecular mass in reduced conditions; after PGNase F treatment, a single band of 45 kDa was observed. The enzymatic kinetic evaluation revealed an allosteric regulation of the enzyme and the kinetic parameters were determined at 37 °C, pH 7.0 as substrate affinity constant, K0.5 = 233 μM, kcat = 54.7 s−1 and Hill coefficient, nH = 1.52, demonstrating positive cooperativity by the enzyme and the substrate. This is the first study to report L. scottii as a source of glutaminase-activity free L-asparaginase, an acute lymphoblastic leukemia drug feature suitable for the treatment of asparagine synthetase negative cancer cells.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConsejo Nacional de Ciencia y Tecnología-
Descrição: dc.descriptionDepartment of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences University of São Paulo, São Paulo-
Descrição: dc.descriptionCentro de Investigación en Biodiversidad para la Salud Universidad Privada Norbert Wiener-
Descrição: dc.descriptionDepartment of Biochemistry Chemistry Institute University of São Paulo, São Paulo-
Descrição: dc.descriptionMarine Biotechnology Division Almirante Paulo Moreira Institute of Studies of the Sea, RJ-
Descrição: dc.descriptionDepartment of General and Applied Biology Biosciences Institute São Paulo State University, São Paulo-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC, São Paulo-
Descrição: dc.descriptionDepartment of General and Applied Biology Biosciences Institute São Paulo State University, São Paulo-
Descrição: dc.descriptionFAPESP: 2013/08617–7-
Descrição: dc.descriptionFAPESP: 2013/19584–2-
Descrição: dc.descriptionFAPESP: 2019/23620–0-
Descrição: dc.descriptionConsejo Nacional de Ciencia y Tecnología: 298596-
Formato: dc.format121-132-
Idioma: dc.languageen-
Relação: dc.relationProcess Biochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntarctic ecosystems-
Palavras-chave: dc.subjectCold-adapted yeast-
Palavras-chave: dc.subjectL-asparaginase-
Palavras-chave: dc.subjectLeucosporidium scottii-
Palavras-chave: dc.subjectLeukemia-
Palavras-chave: dc.subjectPsychrotolerant yeast-
Título: dc.titleAntarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115-
Tipo de arquivo: dc.typelivro digital-
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