Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of Rio de Janeiro-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorOswaldo Cruz Foundation (FIOCRUZ)-
Autor(es): dc.contributorFederal University of Rondonia-
Autor(es): dc.contributorNord University-
Autor(es): dc.contributorSão Leopoldo Mandic-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorInstituto de PesquisasEnergéticas e Nucleares (IPEN-CNEN)-
Autor(es): dc.contributorUniversity Brazil-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.contributorEuropean University at Saint Petersburg-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorFederal University of Santa Maria-
Autor(es): dc.contributorUniversity of Passo Fundo-
Autor(es): dc.contributorGoiano Federal Institute-
Autor(es): dc.creatorCharlie-Silva, Ives-
Autor(es): dc.creatorFeitosa, Natália M.-
Autor(es): dc.creatorPontes, Leticia G.-
Autor(es): dc.creatorFernandes, Bianca H.-
Autor(es): dc.creatorNóbrega, Rafael H.-
Autor(es): dc.creatorGomes, Juliana M. M.-
Autor(es): dc.creatorPrata, Mariana N. L.-
Autor(es): dc.creatorFerraris, Fausto K.-
Autor(es): dc.creatorMelo, Daniela C.-
Autor(es): dc.creatorConde, Gabriel-
Autor(es): dc.creatorRodrigues, Letícia F.-
Autor(es): dc.creatorAracati, Mayumi F.-
Autor(es): dc.creatorCorrêa-Junior, José D.-
Autor(es): dc.creatorManrique, Wilson G.-
Autor(es): dc.creatorSuperio, Joshua-
Autor(es): dc.creatorGarcez, Aguinaldo S.-
Autor(es): dc.creatorConceição, Katia-
Autor(es): dc.creatorYoshimura, Tania M.-
Autor(es): dc.creatorNúñez, Silvia C.-
Autor(es): dc.creatorEto, Silas F.-
Autor(es): dc.creatorFernandes, Dayanne C.-
Autor(es): dc.creatorFreitas, Anderson Z.-
Autor(es): dc.creatorRibeiro, Martha S.-
Autor(es): dc.creatorNedoluzhko, Artem-
Autor(es): dc.creatorLopes-Ferreira, Mônica-
Autor(es): dc.creatorBorra, Ricardo C.-
Autor(es): dc.creatorBarcellos, Leonardo J. G.-
Autor(es): dc.creatorPerez, Andrea C.-
Autor(es): dc.creatorMalafaia, Guilheme-
Autor(es): dc.creatorCunha, Thiago M.-
Autor(es): dc.creatorBelo, Marco A. A.-
Autor(es): dc.creatorGalindo-Villegas, Jorge-
Data de aceite: dc.date.accessioned2025-08-21T16:23:02Z-
Data de disponibilização: dc.date.available2025-08-21T16:23:02Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-09-29-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fimmu.2022.1019201-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248989-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248989-
Descrição: dc.descriptionRegulation of inflammation is a critical process for maintaining physiological homeostasis. The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation. However, the associated mechanisms in vertebrates are not well understood. Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio). We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence. Nevertheless, they generate a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity. Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level. Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN. First, the bioinformatic analysis revealed the composition of the plasma proteome. Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group. Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated. Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group. Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation. Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response. Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Pharmacology University of São Paulo-
Descrição: dc.descriptionIntegrated Laboratory of Translational Bioscience Institute of Biodiversity and Sustainability Federal University of Rio de Janeiro-
Descrição: dc.descriptionDepartment Immunology University of São Paulo-
Descrição: dc.descriptionLaboratório de Controle Genético e Sanitário Faculdade de Medicina Universidade de São Paulo-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Morphology Institute of Biosciences São Paulo State University-
Descrição: dc.descriptionTransplantation Immunobiology Lab Department of Immunology Institute of Biomedical Sciences Universidade de São Paulo-
Descrição: dc.descriptionDepartment of Pharmacology Instituto de CiênciasBiomédicas Universidade Federal de Minas Gerais (ICB-UFMG)-
Descrição: dc.descriptionDepartment of Pharmacology and Toxicology Oswaldo Cruz Foundation (FIOCRUZ)-
Descrição: dc.descriptionLaboratory of Zebrafish from Federal de Minas Gerais (UFMG)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine São Paulo State University-
Descrição: dc.descriptionDepartment of Morphology Instituto de CiênciasBiomédicas-Universidade Federal de Minas Gerais (ICB-UFMG)-
Descrição: dc.descriptionVeterinary College Federal University of Rondonia-
Descrição: dc.descriptionDepartment of Aquaculture Faculty of Biosciences and Aquaculture Nord University-
Descrição: dc.descriptionDepartment of Lasers in Dentistry São Leopoldo Mandic-
Descrição: dc.descriptionPeptide Biochemistry Laboratory Universidade Federal de São Paulo (UNIFESP)-
Descrição: dc.descriptionCenter for Lasers and Applications Instituto de PesquisasEnergéticas e Nucleares (IPEN-CNEN)-
Descrição: dc.descriptionUniversity Brazil-
Descrição: dc.descriptionDevelopment and Innovation Laboratory Center of Innovation and Development Butantan Institute-
Descrição: dc.descriptionPaleogenomics Laboratory European University at Saint Petersburg-
Descrição: dc.descriptionImmunoregulation Unit Butantan Institute-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos-
Descrição: dc.descriptionPostgraduate Program in Pharmacology Federal University of Santa Maria-
Descrição: dc.descriptionUniversity of Passo Fundo-
Descrição: dc.descriptionBiological Research Laboratory Goiano Federal Institute-
Descrição: dc.descriptionCenter of Research in Inflammatory Diseases Ribeirão Preto Medical School University of São Paulo-
Descrição: dc.descriptionDepartment of Pharmacology Ribeirão Preto Medical School University of São Paulo-
Descrição: dc.descriptionDepartment of Genomics Faculty of Biosciences and Aquaculture Nord University-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Morphology Institute of Biosciences São Paulo State University-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine São Paulo State University-
Descrição: dc.descriptionFAPESP: 2013/25971-9-
Descrição: dc.descriptionFAPESP: 2019/19939-1-
Descrição: dc.descriptionCNPq: 301473/2016-1-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectacute-phase proteins-
Palavras-chave: dc.subjectDanio rerio (zebrafish)-
Palavras-chave: dc.subjectglycoproteins-
Palavras-chave: dc.subjectimmunity-
Palavras-chave: dc.subjectmodel organism-
Palavras-chave: dc.subjectoptical coherence tomography (OCT)-
Palavras-chave: dc.subjectproteomics-
Palavras-chave: dc.subjectshotgun LC-MS/MS-
Título: dc.titlePlasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts-
Tipo de arquivo: dc.typelivro digital-
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