Combination of extractive techniques followed by HPLC-MS/MS analysis to monitor ent-agathic acid in fish treated with Copaifera duckei Dwyer

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity de Franca (UNIFRAN)-
Autor(es): dc.creatorda Silva, Jonas Joaquim Mangabeira-
Autor(es): dc.creatorCampanharo, Sarah Chagas-
Autor(es): dc.creatorda Silva, Agnaldo Fernando Baldo-
Autor(es): dc.creatorde Jesus, Raphael Barbetta-
Autor(es): dc.creatorFigueredo, Tais Alexandra Mário-
Autor(es): dc.creatorPilarski, Fabiana-
Autor(es): dc.creatorHeleno, Vladimir Constantino Gomes-
Autor(es): dc.creatorPaschoal, Jonas Augusto Rizzato-
Data de aceite: dc.date.accessioned2025-08-21T16:14:53Z-
Data de disponibilização: dc.date.available2025-08-21T16:14:53Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jchromb.2023.123763-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248893-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248893-
Descrição: dc.descriptionPlants are used as therapeutic alternatives in Veterinary Medicine, including therapies for food-producing animals. However, these medicinal resources can sometimes contain dangerous substances, and when used in animals that supply food, they stand out from the point of view of food safety. The diterpene ent-agathic acid, a component of Copaifera duckei oleoresin, is an example of substances already described with toxic activity in mammals. Thus, this study aimed to propose combining two extractive techniques followed by high-performance liquid chromatography coupled mass spectrometry analysis to monitor residues of ent-agathic acid in Piaractus mesopotamicus fillet treated in an immersion bath with Copaifera duckei oleoresin. An optimized combination of solid–liquid extraction (using acidified acetonitrile) and dispersive liquid–liquid microextraction (using acidified water and chloroform as dispersive and extracting solvent, respectively) was performed to recover the target analyte, added to the development of HPLC-MS/MS method with adequate validation parameters to quantify the ent-agathic acid present in the fish fillet. In vivo tests of residual persistence of ent-agathic acid in fishes treated with C. duckei oleoresin were performed, indicating the non-detection of the target diterpene (< 6.1 µg/mL). The combined extractive procedure followed by quantitative analysis in the in vivo test of residual persistence of the target analyte in fish indicated the absence of ent-agathic acid in all samples. Thus, the data found might contribute to understanding the use of oleoresins from C. duckei as an alternative to traditional veterinary products.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences of Ribeirão Preto Department of Biomolecular Science – University of São Paulo (FCFRP-USP)-
Descrição: dc.descriptionFaculty of Philosophy Sciences and Letters at Ribeirão Preto Department of Chemistry – University of São Paulo (FCFRP-USP)-
Descrição: dc.descriptionLaboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) – São Paulo State University (UNESP)-
Descrição: dc.descriptionNucleus of Research in Sciences and Technology University de Franca (UNIFRAN)-
Descrição: dc.descriptionLaboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) – São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2012/18334-0-
Descrição: dc.descriptionFAPESP: 2017/15052-7-
Descrição: dc.descriptionFAPESP: 2019/06159-8-
Idioma: dc.languageen-
Relação: dc.relationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAquaculture-
Palavras-chave: dc.subjectDLLME-
Palavras-chave: dc.subjectExtraction-
Palavras-chave: dc.subjectFood safety-
Palavras-chave: dc.subjectLC-MS/MS-
Palavras-chave: dc.subjectToxic residue-
Título: dc.titleCombination of extractive techniques followed by HPLC-MS/MS analysis to monitor ent-agathic acid in fish treated with Copaifera duckei Dwyer-
Tipo de arquivo: dc.typelivro digital-
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