EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUNAERP-
Autor(es): dc.creatorPansani, Taisa Nogueira-
Autor(es): dc.creatorBasso, Fernanda Gonçalves-
Autor(es): dc.creatorCardoso, Laís Medeiros-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Data de aceite: dc.date.accessioned2025-08-21T17:24:09Z-
Data de disponibilização: dc.date.available2025-08-21T17:24:09Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jre.13138-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248869-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248869-
Descrição: dc.descriptionObjective: This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF-α). Methods: Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF-α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF-α; G3: Ti + EGF; and G4: Ti + EGF + TNF-α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL-6, IL-8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results: Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions: EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of Dentistry-
Descrição: dc.descriptionDepartment of Dentistry Universidade de Ribeirão Preto UNAERP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of Dentistry-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) Araraquara School of Dentistry-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) Araraquara School of Dentistry-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontal Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectepidermal growth factor-
Palavras-chave: dc.subjectfibroblasts-
Palavras-chave: dc.subjectkeratinocytes-
Palavras-chave: dc.subjecttitanium-
Palavras-chave: dc.subjecttumor necrosis factor alpha-
Título: dc.titleEGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-α-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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