Zinc-Based Nanoparticles Reduce Bacterial Biofilm Formation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Florida College of Dentistry-
Autor(es): dc.contributorFederal Technological University of Paraná (UTFPR)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Florida-
Autor(es): dc.creatorFulindi, Rafael Bianchini-
Autor(es): dc.creatorRodrigues, Juliana Domingues-
Autor(es): dc.creatorBarbosa, Thulio Wliandon Lemos-
Autor(es): dc.creatorGarcia, Ariana D. Goncalves-
Autor(es): dc.creatorDe Almeida La Porta, Felipe-
Autor(es): dc.creatorPratavieira, Sebastião-
Autor(es): dc.creatorChiavacci, Leila Aparecida-
Autor(es): dc.creatorJunior, João Pessoa Araújo-
Autor(es): dc.creatorDa Costa, Paulo Inácio-
Autor(es): dc.creatorMartinez, Luis R.-
Data de aceite: dc.date.accessioned2025-08-21T21:59:46Z-
Data de disponibilização: dc.date.available2025-08-21T21:59:46Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1128/spectrum.04831-22-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248756-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248756-
Descrição: dc.descriptionBiofilm formation is important for microbial survival in hostile environments and a phenotype that provides microorganisms with antimicrobial resistance. Zinc oxide (ZnO) and Zinc sulfide (ZnS) nanoparticles (NPs) present potential antimicrobial properties for biomedical and food industry applications. Here, we aimed to analyze, for the first time, the bactericidal and antibiofilm activity of ZnS NPs against Staphylococcus aureus, Klebsiella oxytoca, and Pseudomonas aeruginosa, all medically important bacteria in developed countries. We compared ZnS NPs antimicrobial activity to ZnO NPs, which have been extensively studied. Using the colorimetric XTT reduction assay to observe the metabolic activity of bacterial cells and the crystal violet assay to measure biofilm mass, we demonstrated that ZnS and ZnO had similar efficacy in killing planktonic bacterial cells and reducing biofilm formation, with S. aureus being more susceptible to both therapeutics than K. oxytoca and P. aeruginosa. Crystal violet staining and confocal microscopy validated that Zn NPs inhibit biofilm formation and cause architectural damage. Our findings provide proof of principle that ZnS NPs have antibiofilm activity, and can be potentially used in medical and food industry applications, such as treatment of wound infections or package coating for food preservation. IMPORTANCE Zinc (Zn)-based nanoparticles (NPs) can be potentially used in medical and food preservation applications. As proof of principle, we investigated the bactericidal and antibiofilm activity of zinc oxide (ZnO) and zinc sulfide (ZnS) NPs against medically important bacteria. Zn-based NPs were similarly effective in killing planktonic and biofilm-Associated Staphylococcus aureus, Klebsiella oxytoca, and Pseudomonas aeruginosa cells. However, S. aureus was more susceptible to these investigational therapeutics. Although further studies are warranted, our findings suggest the possibility of future use of Zn-based NPs in the treatment of skin infections or preservation of food.-
Descrição: dc.descriptionDepartments of Clinical Analysis São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionDepartments of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionDepartment of Oral Biology University of Florida College of Dentistry-
Descrição: dc.descriptionDepartment of Chemistry Federal Technological University of Paraná (UTFPR), Londrina-
Descrição: dc.descriptionSão Carlos Physics Department University of São Paulo, São Carlos-
Descrição: dc.descriptionBiotechnology Institute São Paulo State University, Botucatu-
Descrição: dc.descriptionEmerging Pathogens Institute University of Florida-
Descrição: dc.descriptionCenter for Immunology and Transplantation University of Florida-
Descrição: dc.descriptionCenter for Translational Research in Neurodegenerative Disease University of Florida-
Descrição: dc.descriptionDepartments of Clinical Analysis São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionDepartments of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionBiotechnology Institute São Paulo State University, Botucatu-
Idioma: dc.languageen-
Relação: dc.relationMicrobiology Spectrum-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantimicrobial activity-
Palavras-chave: dc.subjectbacteria-
Palavras-chave: dc.subjectbiofilms-
Palavras-chave: dc.subjectnanoparticles-
Palavras-chave: dc.subjectzinc oxide-
Palavras-chave: dc.subjectzinc sulfide-
Título: dc.titleZinc-Based Nanoparticles Reduce Bacterial Biofilm Formation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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