Covariates of vaginal microbiota and pro-inflammatory cytokine levels in women of reproductive age

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.creatorNovak, J.-
Autor(es): dc.creatorFerreira, C. S.T.-
Autor(es): dc.creatorGolim, M. A.-
Autor(es): dc.creatorSilva, M. G.-
Autor(es): dc.creatorMarconi, C.-
Data de aceite: dc.date.accessioned2025-08-21T21:46:49Z-
Data de disponibilização: dc.date.available2025-08-21T21:46:49Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-18-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3920/BM2022.0060-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248731-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248731-
Descrição: dc.descriptionThis study aimed to assess the correlation between covariates of the vaginal microbiota and local levels of proinflammatory cytokines in women of reproductive age presenting four molecularly defined bacterial community-state types (CSTs). We enrolled 133 non-pregnant women who attended primary care health clinics for routine Pap-testing. Molecular profiling of vaginal microbiota was performed by V3-V4 16S rRNA sequencing. The covariates of vaginal microbiota included were: vaginal pH, total bacterial cell count, diversity (Shannon index), -richness and dominant taxa abundances. Levels of interleukin (IL)-1β, IL-6, IL-8, and tumour necrosis factor (TNF-α) were measured by enzyme-linked immunosorbent assays in supernatants of cervicovaginal fluids. Nonparametric Kruskal-Wallis test was used to compare microbiota covariates and cytokines among different CSTs. Spearman's tests were performed to assess correlations across the measured parameters. A total of 96 (72.2%) participants had CSTs dominated by Lactobacillus spp. (Lactobacillus crispatus CST I, n=38; Lactobacillus gasseri CST II, n=20; and Lactobacillus iners CST III, n=38). A total of 37 (27.8%) presented the Lactobacillus-depleted CST IV. Total bacterial count was higher in CST II (1.29E+05, 3.40E+04-6.69E+05) compared to other Lactobacillus-dominated CSTs (p=0.0003). The highest values of microbiota diversity (1.85; 0.23-2.68) and richness (27.0; 5.0-37.0) were observed in CST IV (P<0.0001). Lower levels of IL-1β were observed in CST I (5.4; 0.0-3,256) when compared to CST III (51.7; 0.0-2,616) and to CST IV (56.2; 0.0-3,407) (P=0.008). Levels of IL-6 were higher in CST II (4.13; 0-131.4) than in CST IV (0.0-58.27) (P=0.02). Correlation tests showed an overall distinct profile of CST II when compared to other Lactobacillusdominated CSTs, particularly regarding the correlation between total bacterial load and cytokines (r>0.39). In conclusion, this study provides evidence of a single pro-inflammatory signature of L. gasseri-dominated microbiota in response to bacterial load. Further studies evaluating a broader range of inflammation markers are warranted.-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, Av. Prof. Mário Rubens Guimarães Montenegro ,s/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Center Blood, Av. Prof. Mário Rubens Guimarães Montenegro ,s/n-
Descrição: dc.descriptionFederal University of Paraná (UFPR) Department of Basic Pathology, Av. Cel. Francisco H. dos Santos-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, Av. Prof. Mário Rubens Guimarães Montenegro ,s/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Center Blood, Av. Prof. Mário Rubens Guimarães Montenegro ,s/n-
Formato: dc.format131-141-
Idioma: dc.languageen-
Relação: dc.relationBeneficial microbes-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject16s rRNA sequencing-
Palavras-chave: dc.subjectbacterial count-
Palavras-chave: dc.subjectbacterial diversity-
Palavras-chave: dc.subjectbacterial vaginosis-
Palavras-chave: dc.subjectLactobacillus spp.-
Título: dc.titleCovariates of vaginal microbiota and pro-inflammatory cytokine levels in women of reproductive age-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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