Condyloma acuminata: An evaluation of the immune response at cellular and molecular levels

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorStuqui, Bruna-
Autor(es): dc.creatorProvazzi, Paola Jocelan Scarin-
Autor(es): dc.creatorLima, Maria Leticia Duarte-
Autor(es): dc.creatorCabral, Ágata Silva-
Autor(es): dc.creatorLeonel, Ellen Cristina Rivas-
Autor(es): dc.creatorCandido, Natalia Maria-
Autor(es): dc.creatorTaboga, Sebastião Roberto-
Autor(es): dc.creatorda Silva, Marcia Guimarães-
Autor(es): dc.creatorde Oliveira Lima, Flávio-
Autor(es): dc.creatorMelli, Patrícia Pereira dos Santos-
Autor(es): dc.creatorQuintana, Silvana Maria-
Autor(es): dc.creatorde Freitas Calmon, Marilia-
Autor(es): dc.creatorRahal, Paula-
Data de aceite: dc.date.accessioned2025-08-21T17:54:16Z-
Data de disponibilização: dc.date.available2025-08-21T17:54:16Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0284296-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248695-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248695-
Descrição: dc.descriptionCondyloma acuminata (CA) is a benign proliferative disease mainly affecting in non-keratinized epithelia. Most cases of CA are caused by low-risk human papillomavirus (HPV), mainly HPV 6 and 11. The aim of the current study was to highlight the candidate genes and pathways associated with immune alterations in individuals who did not spontaneously eliminate the virus and, thus, develop genital warts. Paraffin-embedded condyloma samples (n = 56) were analyzed by immunohistochemistry using antibodies against CD1a, FOXP3, CD3, CD4, CD8, and IFN-γ. The immunomarkers were chosen based on the evaluation of the innate and adaptive immune pathways using qPCR analysis of 92 immune-related genes, applying a TaqMan Array Immune Response assay in HPV 6 or HPV 11 positive samples (n = 27). Gene expression analysis revealed 31 differentially expressed genes in CA lesions. Gene expression validation revealed upregulation of GZMB, IFNG, IL12B, and IL8 and downregulation of NFATC4 and IL7 in CA samples. Immunohistochemical analysis showed increased FOXP3, IFN-γ, CD1a, and CD4 expression in CA than in the control tissue samples. In contrast, CD3 and CD8 expression was decreased in CA lesion samples. Increased levels of pro-inflammatory cytokines in HPV-positive patients compared with HPV-negative patients seem to reflect the elevated immunogenicity of HPV-positive CA lesions. Host defense against HPV begins during the early stages of the innate immune response and is followed by activation of T lymphocytes, which are mainly represented by CD4+ and regulatory T cells. The low CD8+ T cell count in CA may contribute to this recurrent behavior. Additional studies are needed to elucidate the mechanism of host defense against HPV infection in CA.-
Descrição: dc.descriptionDepartment of Biology São Paulo State University, São Josédo Rio Preto-
Descrição: dc.descriptionDepartment of Histology Embryology and Cell Biology Federal University of Goiás, Goiânia-
Descrição: dc.descriptionDepartment of Pathology São Paulo State University, Botucatu-
Descrição: dc.descriptionClinical Hospital of Ribeirao Preto University of São Paulo, Ribeirão Preto-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Ribeirao Preto Medical School University of São Paulo, Ribeirão Preto-
Descrição: dc.descriptionDepartment of Biology São Paulo State University, São Josédo Rio Preto-
Descrição: dc.descriptionDepartment of Pathology São Paulo State University, Botucatu-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleCondyloma acuminata: An evaluation of the immune response at cellular and molecular levels-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.