6-Nitrodopamine potentiates contractions of rat isolated vas deferens induced by noradrenaline, adrenaline, dopamine and electric field stimulation

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorSão Leopoldo Mandic (SLMANDIC)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorBritto-Júnior, José-
Autor(es): dc.creatorLima, Antonio Tiago-
Autor(es): dc.creatorCampos, Rafael-
Autor(es): dc.creatorGião, Alexandre Asturiano-
Autor(es): dc.creatorMendes, Gustavo Duarte-
Autor(es): dc.creatorFerreira-Novaes Silva, Luiza P.-
Autor(es): dc.creatorFregonesi, Adriano-
Autor(es): dc.creatorPupo, André Sampaio-
Autor(es): dc.creatorAntunes, Edson-
Autor(es): dc.creatorDe Nucci, Gilberto-
Data de aceite: dc.date.accessioned2025-08-21T20:57:36Z-
Data de disponibilização: dc.date.available2025-08-21T20:57:36Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00210-023-02478-6-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248625-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248625-
Descrição: dc.description6-Nitrodopamine (6-ND) is a novel endogenous catecholamine that is released from the rat isolated vas deferens, and has been characterized as a major modulator of the contractility of rat isolated epididymal vas deferens (RIEVD). Drugs such as tricyclic antidepressants, α1 and β1β2 adrenoceptor blockers, act as selective antagonists of the 6-ND receptor in the RIEVD. In the rat isolated atria, 6-ND has a potent positive chronotropic action and causes remarkable potentiation of the positive chronotropic effects induced by dopamine, noradrenaline, and adrenaline. Here, whether 6-ND interacts with the classical catecholamines in the rat isolated vas deferens was investigated. Incubation with 6-ND (0.1 and 1 nM; 30min) caused no contractions in the RIEVD but provoked significant leftward shifts in the concentration-response curves to noradrenaline, adrenaline, and dopamine. Pre-incubation of the RIEVD with 6-ND (1 nM), potentiated the contractions induced by electric-field stimulation (EFS), whereas pre-incubation with 1 nM of dopamine, noradrenaline or adrenaline, did not affect EFS-induced contractions. In tetrodotoxin (1 μM) pre-treated (30 min) RIEVD, pre-incubation with 6-ND (0.1 nM) did not cause leftward shifts in the concentration-dependent contractions induced by noradrenaline, adrenaline, or dopamine. Pre-incubation of the RIEVD with the α2A-adrenoceptor antagonist idazoxan (30 min, 10 nM) did not affect dopamine, noradrenaline, adrenaline, and EFS-induced contractions. However, when idazoxan (10 nM) and 6-ND (0.1 nM) were simultaneously pre-incubated (30 min), a significant potentiation of the EFS-induced contractions of the RIEVD was observed. 6-nitrodopamine causes remarkable potentiation of dopamine, noradrenaline, and adrenaline contractions on the RIEVD, due to activation of adrenergic terminals, possibly via pre-synaptic adrenoceptors.-
Descrição: dc.descriptionDepartment of Pharmacology Faculty of Medical Sciences State University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionFaculty of Medicine São Leopoldo Mandic (SLMANDIC), SP-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biomedical Sciences University of São Paulo (USP), SP-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationNaunyn-Schmiedeberg's Archives of Pharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAtropine-
Palavras-chave: dc.subjectIndomethacin-
Palavras-chave: dc.subjectNitrocatecholamines-
Palavras-chave: dc.subjectReserpine-
Palavras-chave: dc.subjectSynergism-
Título: dc.title6-Nitrodopamine potentiates contractions of rat isolated vas deferens induced by noradrenaline, adrenaline, dopamine and electric field stimulation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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