Insulin-like growth factor-1 (IGF-1) selectively modulates the metabolic and lipid profile of bovine embryos according to their kinetics of development

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversité Laval-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAnnes, Kelly-
Autor(es): dc.creatorde Lima, Camila Bruna-
Autor(es): dc.creatorIspada, Jéssica-
Autor(es): dc.creatordos Santos, Érika Cristina-
Autor(es): dc.creatorFontes, Patrícia Kubo-
Autor(es): dc.creatorNichi, Marcílio-
Autor(es): dc.creatorNogueira, Marcelo Fabio Gouveia-
Autor(es): dc.creatorSudano, Mateus José-
Autor(es): dc.creatorMilazzotto, Marcella Pecora-
Data de aceite: dc.date.accessioned2025-08-21T18:11:36Z-
Data de disponibilização: dc.date.available2025-08-21T18:11:36Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2023.03.020-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248621-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248621-
Descrição: dc.descriptionSupplementation of culture media with IGF-1 during in vitro culture of embryos has had controversial results over the years. In the present study, we show that differences previously observed in response to IGF addition might be related to intrinsic heterogeneity of the embryos. In other words, the effects exerted by IGF-1 are dependent on the characteristics of the embryos and their ability to modulate metabolism and overcome stressful conditions, such as the ones found in a non-optimized in vitro culture system. To test this hypothesis, in vitro produced bovine embryos with distinct morphokinetics (fast- and slow-cleavage) were submitted to treatment with IGF-1 and then evaluated for embryo production rates, total cell number, gene expression and lipid profile. Our results show that remarkable differences were found when fast and slow embryos treated with IGF-1 were compared. Fast embryos respond by upregulating genes related to mitochondrial function, stress response, and lipid metabolism, whereas slow embryos presented lower mitochondrial efficiency and lipid accumulation. We conclude that indeed the treatment with IGF-1 selectively affects embryonic metabolism according to early morphokinetics phenotypes, and this information is relevant for decision-making in the design of more appropriate in vitro culture systems.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos, SP-
Descrição: dc.descriptionCentre de Recherche en Reproduction Développement et Santé Intergénérationnelle (CRDSI) Département des Sciences Animales Université Laval-
Descrição: dc.descriptionLaboratory of Embryonic Metabolism and Epigenetic Center of Natural and Human Science Federal University of ABC, SP-
Descrição: dc.descriptionDepartment of Animal Reproduction Faculty of Veterinary Medicine and Animal Science University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University, Campus Assis, SP-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University, Campus Assis, SP-
Descrição: dc.descriptionFAPESP: #16/05986-0-
Descrição: dc.descriptionFAPESP: #17/18384-0-
Formato: dc.format1-7-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine-
Palavras-chave: dc.subjectEmbryos-
Palavras-chave: dc.subjectIGF-
Palavras-chave: dc.subjectLipids-
Palavras-chave: dc.subjectMorphokinetics-
Título: dc.titleInsulin-like growth factor-1 (IGF-1) selectively modulates the metabolic and lipid profile of bovine embryos according to their kinetics of development-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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