Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorOswaldo Cruz Foundation (FIOCRUZ)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Oliveira, Luciana Bonome Zeminian-
Autor(es): dc.creatorDella Coletta, Amanda Manoel-
Autor(es): dc.creatorGardizani, Taiane Priscila-
Autor(es): dc.creatorGarces, Hans Garcia-
Autor(es): dc.creatorBagagli, Eduardo-
Autor(es): dc.creatorTrilles, Luciana-
Autor(es): dc.creatorBarrozo, Ligia Vizeu-
Autor(es): dc.creatorMarques, Sílvio de Alencar-
Autor(es): dc.creatorFaveri, Julio De-
Autor(es): dc.creatorDias-Melicio, Luciane Alarcão-
Data de aceite: dc.date.accessioned2025-08-21T19:14:10Z-
Data de disponibilização: dc.date.available2025-08-21T19:14:10Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms11030562-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248614-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248614-
Descrição: dc.descriptionParacoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: P. brasiliensis (S1), P. americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients’ biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu—UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate Paracoccidioides species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of P. brasiliensis. The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore P. brasiliensis. This is important data, demonstrating the predominance of this species in the São Paulo State region.-
Descrição: dc.descriptionLaboratory of Immunopathology and Infectious Agents—LIAI UNIPEX—Experimental Research Unity—Sector 5 Medical School of Botucatu São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionEvandro Chagas National Institute of Infectious Disease Oswaldo Cruz Foundation (FIOCRUZ), RJ-
Descrição: dc.descriptionDepartment of Geography School of Philosophy Literature and Human Sciences University of São Paulo (USP), SP-
Descrição: dc.descriptionDepartment of Dermatology Medical School of Botucatu São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Pathology Medical School of Botucatu São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Immunopathology and Infectious Agents—LIAI UNIPEX—Experimental Research Unity—Sector 5 Medical School of Botucatu São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Dermatology Medical School of Botucatu São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Pathology Medical School of Botucatu São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectmolecular biology-
Palavras-chave: dc.subjectmolecular phylogenetic analysis-
Palavras-chave: dc.subjectparacoccidiodomycosis-
Palavras-chave: dc.subjectParacoccidioides brasiliensis-
Palavras-chave: dc.subjectParacoccidioides species complex-
Título: dc.titleMolecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples-
Tipo de arquivo: dc.typelivro digital-
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