Influence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversitat de Barcelona-
Autor(es): dc.contributorOmics Animal Biotechnology-
Autor(es): dc.creatorLara, Maria Laura-
Autor(es): dc.creatorCarvalho, Marcos Gomides-
Autor(es): dc.creatorde Souza, Fabiana Ferreira-
Autor(es): dc.creatorSchmith, Rubia Alves-
Autor(es): dc.creatorCodognoto, Viviane Maria-
Autor(es): dc.creatorDe Vita, Bruna-
Autor(es): dc.creatorFreitas Dell'Aqua, Camila de Paula-
Autor(es): dc.creatorLandim, Fernada da Cruz-
Autor(es): dc.creatorAlvarenga, Marina Landim e-
Data de aceite: dc.date.accessioned2025-08-21T20:09:03Z-
Data de disponibilização: dc.date.available2025-08-21T20:09:03Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biochi.2023.03.004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248527-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248527-
Descrição: dc.descriptionThis study aimed to describe the secretome of mesenchymal stem cells derived from feline adipose tissue (AD-MSCs) and compare the effects of different culture conditions on AD-MSC proteomics using a shotgun approach. Adipose tissue was collected from 5 female cats and prepared to culture. Conditioned media was collected at third passage, in which the cells were cultured under 4 conditions, normoxia with fetal bovine serum (N + FBS), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS), and hypoxia without FBS (H – FBS). Then, the secretome was concentrated and prepared for proteomic approaches. Secretomes cultured with FBS-free medium had more than twice identified proteins in comparison with the secretomes cultured with FBS. In contrast, hypoxic conditions did not increase protein amount and affected only a small proteome fraction. Relevant proteins were related to the extracellular matrix promoting environmental modulation, influencing cell signaling pathways, and providing a suitable environment for cell proliferation and maintenance. Moreover, other proteins were also related to cell adhesion, migration and morphogenesis. Culture conditions can influence protein abundance in AD-MSC secretome, and can give also more specificity to cell and cell-free treatments for different diseases.-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University - UNESP, Botucatu-
Descrição: dc.descriptionInternational Product Marketing Manager - PROCARE HEALTH Universitat de Barcelona, Catalunha-
Descrição: dc.descriptionOmics Animal Biotechnology, Botucatu-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University - UNESP, Botucatu-
Formato: dc.format78-86-
Idioma: dc.languageen-
Relação: dc.relationBiochimie-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectConditioned medium-
Palavras-chave: dc.subjectFelis catus-
Palavras-chave: dc.subjectHypoxia-culture-
Palavras-chave: dc.subjectProteomics-
Palavras-chave: dc.subjectSerum-free-culture-
Palavras-chave: dc.subjectStem cell-
Título: dc.titleInfluence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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