LncRNA JHDM1D-AS1 Is a Key Biomarker for Progression and Modulation of Gemcitabine Sensitivity in Bladder Cancer Cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUFOP—Federal University of Ouro Preto-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.contributorFaculdade do Centro Oeste Paulista—FACOP-
Autor(es): dc.contributorUniversidade do Oeste Paulista—UNOESTE-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPereira, Isadora Oliveira Ansaloni-
Autor(es): dc.creatorda Silva, Glenda Nicioli-
Autor(es): dc.creatorAlmeida, Tamires Cunha-
Autor(es): dc.creatorLima, Ana Paula Braga-
Autor(es): dc.creatorSávio, André Luiz Ventura-
Autor(es): dc.creatorLeite, Katia Ramos Moreira-
Autor(es): dc.creatorSalvadori, Daisy Maria Fávero-
Data de aceite: dc.date.accessioned2025-08-21T22:55:09Z-
Data de disponibilização: dc.date.available2025-08-21T22:55:09Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules28052412-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248507-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248507-
Descrição: dc.descriptionLong non-coding RNAs are frequently found to be dysregulated and are linked to carcinogenesis, aggressiveness, and chemoresistance in a variety of tumors. As expression levels of the JHDM1D gene and lncRNA JHDM1D-AS1 are altered in bladder tumors, we sought to use their combined expression to distinguish between low-and high-grade bladder tumors by RTq-PCR. In addition, we evaluated the functional role of JHDM1D-AS1 and its association with the modulation of gemcitabine sensitivity in high-grade bladder-tumor cells. J82 and UM-UC-3 cells were treated with siRNA-JHDM1D-AS1 and/or three concentrations of gemcitabine (0.39, 0.78, and 1.56 µM), and then submitted to cytotoxicity testing (XTT), clonogenic survival, cell cycle progression, cell morphology, and cell migration assays. When JHDM1D and JHDM1D-AS1 expression levels were used in combination, our findings indicated favorable prognostic value. Furthermore, the combined treatment resulted in greater cytotoxicity, a decrease in clone formation, G0/G1 cell cycle arrest, morphological alterations, and a reduction in cell migration capacity in both lineages compared to the treatments alone. Thus, silencing of JHDM1D-AS1 reduced the growth and proliferation of high-grade bladder-tumor cells and increased their sensitivity to gemcitabine treatment. In addition, the expression of JHDM1D/JHDM1D-AS1 indicated potential prognostic value in the progression of bladder tumors.-
Descrição: dc.descriptionPró-Reitoria de Pesquisa e Pós-Graduação, Universidade Federal de Ouro Preto-
Descrição: dc.descriptionUniversidade Federal de Ouro Preto-
Descrição: dc.descriptionDepartamento de Análises Clínicas Pharmacy School UFOP—Federal University of Ouro Preto, MG-
Descrição: dc.descriptionLaboratory of Pain and Signaling Butantan Institute, SP-
Descrição: dc.descriptionDepartamento de Odontologia Faculdade do Centro Oeste Paulista—FACOP, SP-
Descrição: dc.descriptionDepartamento de Ciências Médicas Universidade do Oeste Paulista—UNOESTE, SP-
Descrição: dc.descriptionDepartamento de Cirurgia Medical School USP—University of São Paulo, SP-
Descrição: dc.descriptionDepartamento de Patologia Medical School UNESP—São Paulo State University, SP-
Descrição: dc.descriptionDepartamento de Patologia Medical School UNESP—São Paulo State University, SP-
Descrição: dc.descriptionPró-Reitoria de Pesquisa e Pós-Graduação, Universidade Federal de Ouro Preto: PROPPI/UFOP Nº 03/2023-
Descrição: dc.descriptionUniversidade Federal de Ouro Preto: PROPPI/UFOP Nº 03/2023-
Idioma: dc.languageen-
Relação: dc.relationMolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbladder cancer-
Palavras-chave: dc.subjectJHDM1D-AS1-
Palavras-chave: dc.subjectlong non-coding RNAs-
Título: dc.titleLncRNA JHDM1D-AS1 Is a Key Biomarker for Progression and Modulation of Gemcitabine Sensitivity in Bladder Cancer Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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