Apelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity Medical Center of the Johannes Gutenberg University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Bern-
Autor(es): dc.contributorEge University-
Autor(es): dc.creatorCores Ziskoven, Pablo-
Autor(es): dc.creatorNogueira, Andressa V. B.-
Autor(es): dc.creatorGutierrez, Lorena S.-
Autor(es): dc.creatorWeusmann, Jens-
Autor(es): dc.creatorEick, Sigrun-
Autor(es): dc.creatorBuduneli, Nurcan-
Autor(es): dc.creatorDeschner, James-
Data de aceite: dc.date.accessioned2025-08-21T16:58:40Z-
Data de disponibilização: dc.date.available2025-08-21T16:58:40Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms24054733-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248500-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248500-
Descrição: dc.descriptionThis study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis.-
Descrição: dc.descriptionGerman-Israeli Foundation for Scientific Research and Development-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst-
Descrição: dc.descriptionDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionLaboratory of Oral Microbiology Department of Periodontology University of Bern-
Descrição: dc.descriptionDepartment of Periodontology School of Dentistry Ege University-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP-
Descrição: dc.descriptionGerman-Israeli Foundation for Scientific Research and Development: 1593/5-1-
Descrição: dc.descriptionDeutscher Akademischer Austauschdienst: 57589445-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectapelin-
Palavras-chave: dc.subjectAPJ-
Palavras-chave: dc.subjectFusobacterium nucleatum-
Palavras-chave: dc.subjectobesity-
Palavras-chave: dc.subjectperiodontal ligament cells-
Palavras-chave: dc.subjectperiodontitis-
Título: dc.titleApelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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