An Easy-to-Use Histological Technique for Small Biological Samples of Senegalese Sole Larvae

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Aveiro-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIPMA—Portuguese Institute for the Ocean and Atmosphere-
Autor(es): dc.creatorSantana, Lígia M. B. M.-
Autor(es): dc.creatorDamasceno, Évila Pinheiro-
Autor(es): dc.creatorLoureiro, Susana-
Autor(es): dc.creatorSoares, Amadeu M. V. M.-
Autor(es): dc.creatorPousão-Ferreira, Pedro-
Autor(es): dc.creatorAbessa, Denis M. S.-
Autor(es): dc.creatorMartins, Roberto-
Autor(es): dc.creatorPavlaki, Maria D.-
Data de aceite: dc.date.accessioned2025-08-21T20:42:54Z-
Data de disponibilização: dc.date.available2025-08-21T20:42:54Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/app13042346-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248454-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248454-
Descrição: dc.descriptionHistological techniques are a traditional and essential tool for adequately comprehending cellular morphology and physiology in several scientific fields, including ecotoxicological studies. Although bioassays generally recommend using small test organisms, based on advantages such as low maintenance costs and the possibility of a higher number of replicates, the histological process of such organisms is still challenging due to the difficulty of handling them. The present study proposes an accessible protocol for performing the histology of a biological sample of 8 mm maximum length and using histopathological biomarkers in Senegal sole larvae for ecotoxicological tests. Eight-day old larvae of Solea senegalensis obtained from breeders’ natural spawns in cultivation conditions were submitted to histological processing by an adjusted protocol comprising fixation, agarose array pre-embedding, dehydration, paraffin embedding, slides’ preparation, and image analysis by light optical microscope coupled to a digital camera. Low-cost and easy-to-use alternatives were applied in the technique execution. The histological steps were detailed, and S. senegalensis morphological features were presented. This can be considered valuable background information for ecotoxicological studies where changes in cell morphology and physiology are expected.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCESAM-Centre for Environmental and Marine Studies Department of Biology University of Aveiro-
Descrição: dc.descriptionNEPEA-Núcleo de Estudos em Poluição e Ecotoxicologia Aquática São Paulo State University, Campus do Litoral Paulista, SP-
Descrição: dc.descriptionEPPO-Aquaculture Research Station IPMA—Portuguese Institute for the Ocean and Atmosphere, Av. Parque Natural da Ria Formosa s/n-
Descrição: dc.descriptionNEPEA-Núcleo de Estudos em Poluição e Ecotoxicologia Aquática São Paulo State University, Campus do Litoral Paulista, SP-
Descrição: dc.descriptionCNPq: 308533/2018-6-
Idioma: dc.languageen-
Relação: dc.relationApplied Sciences (Switzerland)-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectagarose pre-embedding-
Palavras-chave: dc.subjectfish larvae-
Palavras-chave: dc.subjecthistology-
Palavras-chave: dc.subjecthistopathological biomarker-
Palavras-chave: dc.subjectsmall samples-
Título: dc.titleAn Easy-to-Use Histological Technique for Small Biological Samples of Senegalese Sole Larvae-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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