Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.contributorGuarulhos University (UnG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Pittsburgh-
Autor(es): dc.contributorDental Science School (Faculdade de Ciências Odontológicas - FCO)-
Autor(es): dc.creatorMiranda, Luis Fernando B.-
Autor(es): dc.creatorLima, Carolina V.-
Autor(es): dc.creatorPagin, Rafaela-
Autor(es): dc.creatorCosta, Raphael C.-
Autor(es): dc.creatorPereira, Marta Maria A.-
Autor(es): dc.creatorde Avila, Erica D.-
Autor(es): dc.creatorBertolini, Martinna-
Autor(es): dc.creatorRetamal-Valdes, Belén-
Autor(es): dc.creatorShibli, Jamil A.-
Autor(es): dc.creatorFeres, Magda-
Autor(es): dc.creatorBarão, Valentim A. R.-
Autor(es): dc.creatorSouza, João Gabriel S.-
Data de aceite: dc.date.accessioned2025-08-21T20:01:00Z-
Data de disponibilização: dc.date.available2025-08-21T20:01:00Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-03-03-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.jproteome.2c00652-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248374-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248374-
Descrição: dc.descriptionThe use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP), Sa̅o Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry Federal University of Paraná (UFPR), Paraná-
Descrição: dc.descriptionDepartment of Periodontology Dental Research Division Guarulhos University (UnG), Sa̅o Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Periodontics and Preventive Dentistry School of Dental Medicine University of Pittsburgh-
Descrição: dc.descriptionDental Science School (Faculdade de Ciências Odontológicas - FCO), Minas Gerais-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), São Paulo-
Formato: dc.format857-870-
Idioma: dc.languageen-
Relação: dc.relationJournal of Proteome Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectmicrobiology-
Palavras-chave: dc.subjectproteomic-
Palavras-chave: dc.subjectsaliva-
Palavras-chave: dc.subjectsterilization-
Palavras-chave: dc.subjectstudy model-
Título: dc.titleEffect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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