Assessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDe Oliveira Gallinari, Marjorie-
Autor(es): dc.creatorBordini, Ester Alves Ferreira-
Autor(es): dc.creatorDe Toledo Stuani, Vitor-
Autor(es): dc.creatorCassiano, Fernanda Balestrero-
Autor(es): dc.creatorDe Melo, Camila Correa Da Silva Braga-
Autor(es): dc.creatorDe Almeida, Juliano Milanezi-
Autor(es): dc.creatorCintra, Luciano Tavares Ângelo-
Autor(es): dc.creatorDe Souza Costa, Carlos Alberto-
Autor(es): dc.creatorSoares, Diana Gabriela-
Data de aceite: dc.date.accessioned2025-08-21T21:04:50Z-
Data de disponibilização: dc.date.available2025-08-21T21:04:50Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1807-3107BOR-2023.VOL37.0018-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248370-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248370-
Descrição: dc.descriptionThis study evaluated the bioactive potential of a macro-porous chitosan scaffold incorporated with calcium hydroxide (CH-Ca) and functionalized with bioactive doses of simvastatin (SV) for bone tissue regeneration. Initially, the bioactive dose of SV in osteoblastic cells (SAOS-2) was determined. For the direct contact experiment, SAOS-2 cells were plated on scaffolds to assess cell viability and osteogenic differentiation. The second assay was performed at a distance using extracts from scaffolds incubated in culture medium to assess the effect of conditioned medium on viability and osteogenic differentiation. The initial screening showed that 1 μM SV presented the best biostimulating effects, and this dose was selected for incorporation into the CH-Ca and pure chitosan (CH) scaffolds. The cells remained viable throughout the direct contact experiment, with the greatest cell density in the CH-Ca and CH-Ca-SV scaffolds because of their higher porosity. The CH-Ca-SV scaffold showed the most intense bio-stimulating effect in assays in the presence and absence of osteogenic medium, leading to an increased deposition of mineralized matrix. There was an increase in the viability of cells exposed to the extracts for CH-Ca, CH-SV, and CH-Ca-SV during the one-day period. There was an increase in ALP activity in the CH-Ca and CH-Ca-SV; however, the CH-Ca-SV scaffold resulted in an intense increase in the deposition of mineralized nodules, approximately 56.4% at 7 days and 117% at 14 days, compared with CH (control). In conclusion, functionalization of the CH-Ca scaffold with SV promoted an increase in bioactivity, presenting a promising option for bone tissue regeneration-
Descrição: dc.descriptionUniversidade de São Paulo USP Bauru School of Dentistry Department of Operative Dentistry Endodontics and Dental Materials, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Diagnosis and Surgery, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Preventive and Operative Dentistry, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araraquara School of Dentistry Department of Physiology and Pathology, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Diagnosis and Surgery, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Preventive and Operative Dentistry, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Unesp Araraquara School of Dentistry Department of Physiology and Pathology, SP-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Oral Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectSimvastatin-
Palavras-chave: dc.subjectTissue Engineering-
Título: dc.titleAssessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.