Extracellular lipids of Candida albicans biofilm induce lipid droplet formation and decreased response to a topoisomerase I inhibitor in dysplastic and neoplastic oral cells

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de Araraquara (UNIARA)-
Autor(es): dc.creatorMarin-Dett, Freddy Humberto-
Autor(es): dc.creatorCampanella, Jonatas Erick Maimoni-
Autor(es): dc.creatorTrovatti, Eliane-
Autor(es): dc.creatorBertolini, Maria Célia-
Autor(es): dc.creatorVergani, Carlos Eduardo-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Data de aceite: dc.date.accessioned2025-08-21T19:23:12Z-
Data de disponibilização: dc.date.available2025-08-21T19:23:12Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2022-0319-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248333-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248333-
Descrição: dc.descriptionObjective: Some microorganisms, i.e., Candida albicans, have been associated with cancer onset and development, although whether the fungus promotes cancer or whether cancer facilitates the growth of C. albicans is unclear. In this context, microbial-derived molecules can modulate the growth and resistance of cancer cells. This study isolated extracellular lipids (ECL) from a 36-h Candida albicans biofilm incubated with oral dysplastic (DOK) and neoplastic (SCC 25) cells, which were further challenged with the topoisomerase I inhibitor camptothecin (CPT), a lipophilic anti-tumoral molecule. Methodology: ECL were extracted from a 36-h Candida albicans biofilm with the methanol/chloroform precipitation method and identified with Nuclear Magnetic Resonance (1H-NMR). The MTT tetrazolium assay measured ECL cytotoxicity in DOK and SCC 25 cells, alamarBlue™ assessed cell metabolism, flow cytometry measured cell cycle, and confocal microscopy determined intracellular features. Results: Three major classes of ECL of C. albicans biofilm were found: phosphatidylinositol (PI), phosphatidylcholine (PC), and phosphatidylglycerol (PG). The ECL of C. albicans biofilm had no cytotoxic effect on neither cell after 24 hours, with a tendency to disturb the SCC 25 cell cycle profile (without statistical significance). The ECL-induced intracellular lipid droplet (LD) formation on both cell lines after 72 hours. In this context, ECL enhanced cell metabolism, decreased the response to CPT, and modified intracellular drug distribution. Conclusion: The ECL (PI, PC, and PG) of 36-h Candida albicans biofilm directly interacts with dysplastic and neoplastic oral cells, highlighting the relevance of better understanding C. albicans biofilm signaling in the microenvironment of tumor cells.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas-
Descrição: dc.descriptionUniversidade de Araraquara (UNIARA) Departamento de Saúde e Ciências Biológicas-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Oral Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectLipids-
Palavras-chave: dc.subjectOral cancer-
Título: dc.titleExtracellular lipids of Candida albicans biofilm induce lipid droplet formation and decreased response to a topoisomerase I inhibitor in dysplastic and neoplastic oral cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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