Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase

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Autor(es): dc.contributorUniversity of Sheffield-
Autor(es): dc.contributorThe University of Texas Medical Branch at Galveston-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorThe University of Sheffield-
Autor(es): dc.creatorAmabebe, Emmanuel-
Autor(es): dc.creatorRichardson, Lauren S.-
Autor(es): dc.creatorBento, Giovana Fernanda Cosi-
Autor(es): dc.creatorRadnaa, Enkhtuya-
Autor(es): dc.creatorKechichian, Talar-
Autor(es): dc.creatorMenon, Ramkumar-
Autor(es): dc.creatorAnumba, Dilly O. C.-
Data de aceite: dc.date.accessioned2025-08-21T18:37:50Z-
Data de disponibilização: dc.date.available2025-08-21T18:37:50Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11033-022-08183-6-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248230-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248230-
Descrição: dc.descriptionBackground: Ureaplasma, a genus of the order Mycoplasmatales and commonly grouped with Mycoplasma as genital mycoplasma is one of the most common microbes isolated from women with infection/inflammation-associated preterm labor (PTL). Mycoplasma spp. produce sialidase that cleaves sialic acid from glycans of vaginal mucous membranes and facilitates adherence and invasion of the epithelium by pathobionts, and dysregulated immune response. However, whether Ureaplasma species can induce the production of sialidase is yet to be demonstrated. We examined U. parvum-infected vaginal epithelial cells (VECs) for the production of sialidase and pro-inflammatory cytokines. Methods: Immortalized VECs were cultured in appropriate media and treated with U. parvum in a concentration of 1 × 105 DNA copies/ml. After 24 h of treatment, cells and media were harvested. To confirm infection and cell uptake, immunocytochemistry for multi-banded antigen (MBA) was performed. Pro-inflammatory cytokine production and protein analysis for sialidase confirmed pro-labor pathways. Results: Infection of VECs was confirmed by the presence of intracellular MBA. Western blot analysis showed no significant increase in sialidase expression from U. parvum-treated VECs compared to uninfected cells. However, U. parvum infection induced 2-3-fold increased production of GM-CSF (p = 0.03), IL-6 (p = 0.01), and IL-8 (p = 0.01) in VECs compared to controls. Conclusion: U. parvum infection of VECs induced inflammatory imbalance associated with vaginal dysbiosis but did not alter sialidase expression at the cellular level. These data suggest that U. parvum’s pathogenic effect could be propagated by locally produced pro-inflammatory cytokines and, unlike other genital mycoplasmas, may be independent of sialidase.-
Descrição: dc.descriptionDepartment of Oncology and Metabolism University of Sheffield-
Descrição: dc.descriptionDepartment of Obstetrics and Gynecology The University of Texas Medical Branch at Galveston-
Descrição: dc.descriptionDepartment of Pathology Universidade Estadual Paulista Botucatu Medical School-
Descrição: dc.descriptionAcademic Unit of Reproductive and Developmental Medicine Department of Oncology and Metabolism The University of Sheffield, 4th Floor, Jessop Wing, Tree Root Walk-
Descrição: dc.descriptionDepartment of Obstetrics & Gynecology The University of Texas Medical Branch at Galveston, 301 University Blvd-
Descrição: dc.descriptionDepartment of Pathology Universidade Estadual Paulista Botucatu Medical School-
Formato: dc.format3035-3043-
Idioma: dc.languageen-
Relação: dc.relationMolecular Biology Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectPreterm birth-
Palavras-chave: dc.subjectSialidase-
Palavras-chave: dc.subjectUreaplasma parvum-
Palavras-chave: dc.subjectVaginal epithelial cells-
Título: dc.titleUreaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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