Unravelling the dorsal periaqueductal grey matter NMDA receptors relevance in the nitric oxide-mediated panic‑like behaviour and defensive antinociception organised by the anterior hypothalamus of male mice

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBehavioural Neurosciences Institute (INeC)-
Autor(es): dc.contributorBiomedical Sciences Institute of the Federal University of Alfenas (UNIFAL)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFalconi-Sobrinho, Luiz Luciano-
Autor(es): dc.creatordos Anjos-Garcia, Tayllon-
Autor(es): dc.creatorHernandes, Paloma Molina-
Autor(es): dc.creatorRodrigues, Bruno Mangili de Paula-
Autor(es): dc.creatorAlmada, Rafael Carvalho-
Autor(es): dc.creatorCoimbra, Norberto Cysne-
Data de aceite: dc.date.accessioned2025-08-21T21:43:25Z-
Data de disponibilização: dc.date.available2025-08-21T21:43:25Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00213-023-06309-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248215-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248215-
Descrição: dc.descriptionRationale: Previous studies suggested that the dorsal column of the periaqueductal grey matter (dPAG) can be a target of neural pathways from hypothalamic nuclei involved in triggering fear-related defensive responses. In turn, evidence is provided suggesting that microinjection of the nitric oxide (NO) donor SIN-1 into the anterior hypothalamus (AH) of mice evokes panic-like behaviours and fear-induced antinociception. However, it is unknown whether the dPAG of mice mediates these latter defensive responses organised by AH neurons. Objectives: This study was designed to examine the role of dPAG in mediating SIN-1-evoked fear-induced defensive behavioural and antinociceptive responses organised in the AH of mice. Methods: First, neural tract tracing was performed to characterise the AH-dPAG pathways. Then, using neuropharmacological approaches, we evaluated the effects of dPAG pretreatment with either the non-selective synaptic blocker cobalt chloride (CoCl2; 1 mM/0.1 μL) or the competitive N-methyl-d-aspartate (NMDA) receptor antagonist LY235959 (0.1 nmol/0.1 μL) on defensive behaviours and antinociception induced by microinjections of SIN-1 in the AH of male C57BL/6 mice. Results: AlexaFluor488-conjugated dextran-labelled axonal fibres from AH neurons were identified in both dorsomedial and dorsolateral PAG columns. Furthermore, we showed that pre-treatment of the dPAG with either CoCl2 or LY235959 inhibited freezing and impaired oriented escape and antinociception induced by infusions of SIN-1 into the AH. Conclusions: These findings suggest that the panic-like freezing and oriented escape defensive behaviours, and fear-induced antinociception elicited by intra-AH microinjections of SIN-1 depend on the activation of dPAG NMDA receptors.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Neuroanatomy and Neuropsychobiology Department of Pharmacology Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes 3900, Ribeirão Preto-
Descrição: dc.descriptionNAP-USP-Neurobiology of Emotions (NuPNE) Research Centre Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes 3900, Ribeirão Preto-
Descrição: dc.descriptionBehavioural Neurosciences Institute (INeC), Avenida do Café, 2450, Ribeirão Preto-
Descrição: dc.descriptionBiomedical Sciences Institute of the Federal University of Alfenas (UNIFAL), Minas Gerais-
Descrição: dc.descriptionDepartment of Biological Sciences School of Science Humanities and Languages São Paulo State University (UNESP), Assis-
Descrição: dc.descriptionDepartment of Biological Sciences School of Science Humanities and Languages São Paulo State University (UNESP), Assis-
Descrição: dc.descriptionFAPESP: 2007/01174-1-
Descrição: dc.descriptionFAPESP: 2012/03798-0-
Descrição: dc.descriptionFAPESP: 2017/11855-8-
Descrição: dc.descriptionFAPESP: 2020/15050-7-
Formato: dc.format319-335-
Idioma: dc.languageen-
Relação: dc.relationPsychopharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnterior hypothalamus-
Palavras-chave: dc.subjectDefensive behaviour-
Palavras-chave: dc.subjectFear-induced analgesia-
Palavras-chave: dc.subjectNitric oxide-
Palavras-chave: dc.subjectNMDA receptors-
Palavras-chave: dc.subjectPeriaqueductal grey matter-
Palavras-chave: dc.subjectStress-
Título: dc.titleUnravelling the dorsal periaqueductal grey matter NMDA receptors relevance in the nitric oxide-mediated panic‑like behaviour and defensive antinociception organised by the anterior hypothalamus of male mice-
Tipo de arquivo: dc.typelivro digital-
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