The Neurospora crassa PCL-1 cyclin is a PHO85-1 (PGOV) kinase partner that directs the complex to glycogen metabolism and is involved in calcium metabolism regulation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Itajubá-
Autor(es): dc.creatorCampanella, Jonatas Erick Maimoni-
Autor(es): dc.creatorCandido, Thiago de Souza-
Autor(es): dc.creatorBarbosa, Luiz Carlos Bertucci-
Autor(es): dc.creatorGomes, Antoniel Augusto Severo-
Autor(es): dc.creatorLeite, Carla Andréa-
Autor(es): dc.creatorHigashi, Erika Silva-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Autor(es): dc.creatorFontes, Marcos Roberto de Matos-
Autor(es): dc.creatorBertolini, Maria Célia-
Data de aceite: dc.date.accessioned2025-08-21T22:14:49Z-
Data de disponibilização: dc.date.available2025-08-21T22:14:49Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-21-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fmicb.2022.1078972-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248150-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248150-
Descrição: dc.descriptionCyclins are a family of proteins characterized by possessing a cyclin box domain that mediates binding to cyclin dependent kinases (CDKs) partners. In this study, the search for a partner cyclin of the PHO85-1 CDK retrieved PCL-1 an ortholog of yeast Pcls (for Pho85 cyclins) that performs functions common to Pcls belonging to different cyclin families. We show here that PCL-1, as a typical cyclin, is involved in cell cycle control and cell progression. In addition, PCL-1 regulates glycogen metabolism; Δpcl-1 cells accumulate higher glycogen levels than wild-type cells and the glycogen synthase (GSN) enzyme is less phosphorylated and, therefore, more active in the mutant cells. Together with PHO85-1, PCL-1 phosphorylates in vitro GSN at the Ser636 amino acid residue. Modeling studies identified PHO85-1 and PCL-1 as a CDK/cyclin complex, with a conserved intermolecular region stabilized by hydrophobic and polar interactions. PCL-1 is also involved in calcium and NaCl stress response. Δpcl-1 cells are sensitive to high NaCl concentration; on the contrary, they grow better and overexpress calcium responsive genes under high calcium chloride concentration compared to the wild-type strain. The expression of the calcium-responsive CRZ-1 transcription factor is modulated by PCL-1, and this transcription factor seems to be less phosphorylated in Δpcl-1 cells since exhibits nuclear location in these cells in the absence of calcium. Our results show that PCL-1 locates at different cell regions suggesting that it may determine its activity by controlling its intracellular location and reveal an interesting functional divergence between yeast and filamentous fungus cyclins.-
Descrição: dc.descriptionDepartamento de Bioquímica e Química Orgânica Instituto de Química Universidade Estadual Paulista, São Paulo-
Descrição: dc.descriptionDepartamento de Biofísica e Farmacologia Instituto de Biociências Universidade Estadual Paulista, São Paulo-
Descrição: dc.descriptionDepartamento de Materiais Dentários e Prótese Faculdade de Odontologia Universidade Estadual Paulista, São Paulo-
Descrição: dc.descriptionInstituto de Recursos Naturais Universidade Federal de Itajubá, Minas Gerais-
Descrição: dc.descriptionDepartamento de Bioquímica e Química Orgânica Instituto de Química Universidade Estadual Paulista, São Paulo-
Descrição: dc.descriptionDepartamento de Biofísica e Farmacologia Instituto de Biociências Universidade Estadual Paulista, São Paulo-
Descrição: dc.descriptionDepartamento de Materiais Dentários e Prótese Faculdade de Odontologia Universidade Estadual Paulista, São Paulo-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCDK/cyclin complex-
Palavras-chave: dc.subjectCRZ-1 transcription factor-
Palavras-chave: dc.subjectNeurospora crassa-
Palavras-chave: dc.subjectprotein phosphorylation-
Palavras-chave: dc.subjectstress response-
Título: dc.titleThe Neurospora crassa PCL-1 cyclin is a PHO85-1 (PGOV) kinase partner that directs the complex to glycogen metabolism and is involved in calcium metabolism regulation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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