Regenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Araraquara—UNIARA-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorClaro, Amanda Maria-
Autor(es): dc.creatorAlves, Caroline Cássia-
Autor(es): dc.creatordos Santos, Kelvin Sousa-
Autor(es): dc.creatorda Rocha, Euzane Gomes-
Autor(es): dc.creatorde Lima Fontes, Marina-
Autor(es): dc.creatorMonteiro, Gustavo Claro-
Autor(es): dc.creatorde Carvalho, Gustavo Senra Gonçalves-
Autor(es): dc.creatorCaiut, José Maurício Almeida-
Autor(es): dc.creatorMoroz, Andrei-
Autor(es): dc.creatorRibeiro, Sidney José Lima-
Autor(es): dc.creatorS. Barud, Hernane-
Data de aceite: dc.date.accessioned2025-08-21T22:34:30Z-
Data de disponibilização: dc.date.available2025-08-21T22:34:30Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10971-022-05990-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248119-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248119-
Descrição: dc.descriptionTissue engineering has emerged as a multidisciplinary field that aims to improve health and quality of life by restoring functions of tissues and organs. Cells and scaffolds are the two major components of tissue engineering. Scaffolds act as a support for cells, thus facilitating cell adhesion, proliferation, morphogenesis, differentiation, and extracellular matrix production. Since three-dimensional (3D) porous scaffolds can better simulate the native 3D architecture of in vivo systems than conventional 2D cultures, they are more appropriate to support tissue regeneration. This study aimed to use regenerated cellulose sponge (RCS) as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold (ASS). RCS was coated with boehmite-GPTS (3-glycidoxypropyltrimethoxysilane) solution and dried at 60 °C for 12 h. The coated RCS was converted into porous alumina-silica scaffold via thermal treatment at 500 °C for 4 h in air. The materials were characterized by Fourier transform‐infrared (FT‐IR), X-Ray diffraction (XRD), thermogravimetric analysis (TGA) aluminum-27 and silicon-29 nuclear magnetic resonance (NMR), scanning electron microscopy, and energy dispersive X-ray spectroscopy (SEM-EDS). The MTT metabolism assays were used to evaluate indirectly cytocompatibility and cell proliferation using MCT3T3-E1, HDFa and HaCaT cells. The biological in vitro assays demonstrated that only RCS exhibited toxicity toward HDFa cells, although this behavior has been shown questionable once other studies have reported the non-cytotoxic, mutagenic and genotoxic potential of RCS. We believe that tests including clonogenic and mutagenic assays should be performed using HDFa cells in contact with RCS-derived extract in order to further investigate this behavior. The sponge materials have demonstrated different growth rate and adhesion to three different cell lines evaluated. Therefore, the employed sacrificial template approach presents as a viable alternative to produce 3D scaffolds for tissue engineering. Graphical abstract: [Figure not available: see fulltext.]-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversity of Araraquara—UNIARA, Rua Carlos Gomes 1217, SP-
Descrição: dc.descriptionDepartment of Chemistry Faculdade de Filosofia Ciências e Letras de Ribeirão Preto University of São Paulo, SP-
Descrição: dc.descriptionThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SP-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SP-
Descrição: dc.descriptionThe School of Pharmaceutical Sciences—FCF Department of Clinical Analysis São Paulo State University—UNESP, Rodovia Araraquara-Jaú, KM 01, SP-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University—UNESP, Rua Prof. Francisco Degni 55, SP-
Formato: dc.format83-95-
Idioma: dc.languageen-
Relação: dc.relationJournal of Sol-Gel Science and Technology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAlumina-silica-
Palavras-chave: dc.subjectSacrificial template-
Palavras-chave: dc.subjectScaffold-
Palavras-chave: dc.subjectTissue engineering-
Título: dc.titleRegenerated cellulose sponge as sacrificial template for the synthesis of three-dimensional porous alumina-silica scaffold for tissue engineering-
Tipo de arquivo: dc.typelivro digital-
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