Melatonin decreases IRF-3 protein expression in the gastrocnemius muscle, reduces IL-1β and LPS plasma concentrations, and improves the lipid profile in rats with apical periodontitis fed on a high-fat diet

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatordos Santos, Rodrigo Martins-
Autor(es): dc.creatorBelardi, Bianca Elvira-
Autor(es): dc.creatorTsosura, Thais Verônica Saori-
Autor(es): dc.creatorChiba, Fernando Yamamoto-
Autor(es): dc.creatorMattera, Maria Sara de Lima Coutinho-
Autor(es): dc.creatorMachado, Nathalia Evelyn Silva-
Autor(es): dc.creatorCantiga-Silva, Cristiane-
Autor(es): dc.creatorCarvalho, Núbia Ramos-
Autor(es): dc.creatorBravo, Lara Teschi-
Autor(es): dc.creatorNobumoto, Ana Carla Thalez Ywabuchi-
Autor(es): dc.creatorOliveira, Sandra Helena Penha de-
Autor(es): dc.creatorCintra, Luciano Tavares Angelo-
Autor(es): dc.creatorMatsushita, Dóris Hissako-
Data de aceite: dc.date.accessioned2025-08-21T18:28:37Z-
Data de disponibilização: dc.date.available2025-08-21T18:28:37Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10266-022-00782-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248092-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248092-
Descrição: dc.descriptionTo evaluate the effects of melatonin (MEL) on the expression of toll-like receptor-4 (TLR4); myeloid differentiation primary response protein-88 (MyD88); TIR-domain-containing adapter-inducing interferon-β (TRIF); IFN regulatory-factor-3 (IRF-3); nuclear factor kappa-B (NF-κB); plasma concentrations of interleukin-1β (IL-1β) and lipopolysaccharide (LPS); and lipid profile of rats with apical periodontitis (AP) fed on a high-fat diet (HFD). Eighty 60-day-old rats were divided into eight groups: control, AP, HFD, HFDAP, CNMEL, APMEL, HFDMEL and HFDAPMEL. HFD groups were fed on a HFD for 107 days. On day 7, experimental AP was induced in the AP groups, and after 70 days, MEL (5 mg/kg) was administered to the MEL groups for 30 days. Plasma concentrations of LPS and IL-1β were analyzed using enzyme-linked immunosorbent assay, and the lipid profile was analyzed using biochemical tests. The expression of proteins involved in the TLR4 pathway (TLR4, MyD88, TRIF, IRF-3 and NF-κB) in the gastrocnemius muscle (GM) was evaluated using western blotting and qRT-PCR. Treatment with MEL decreased IRF-3 protein expression in GM and IL-1β plasma concentration in the APMEL and HFDMEL groups. Reduction in LPS plasma concentration was reported only in the HFDMEL group. Additionally, a decrease in LDL and an increase in HDL were observed in the HFDMEL and HFDAPMEL groups. Treatment with MEL exhibited anti-inflammatory and anti-hyperlipidemic effects attributed to HFD and AP by reducing the plasma concentrations of IL-1β and LPS in addition to reducing IRF-3 protein expression in the GM, which is associated with the production of inflammatory cytokines.-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Basic Sciences São Paulo State University (UNESP), R. José Bonifácio, 1193-
Descrição: dc.descriptionPrograma de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF) Sociedade Brasileira de Fisiologia (SBFis) School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Basic Sciences São Paulo State University (UNESP), R. José Bonifácio, 1193-
Descrição: dc.descriptionPrograma de Pós-Graduação Multicêntrico Em Ciências Fisiológicas (PPGMCF) Sociedade Brasileira de Fisiologia (SBFis) School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (UNESP), SP-
Formato: dc.format687-696-
Idioma: dc.languageen-
Relação: dc.relationOdontology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApical periodontitis-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectHigh-fat diet-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectMelatonin-
Palavras-chave: dc.subjectToll-like receptor-
Título: dc.titleMelatonin decreases IRF-3 protein expression in the gastrocnemius muscle, reduces IL-1β and LPS plasma concentrations, and improves the lipid profile in rats with apical periodontitis fed on a high-fat diet-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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