Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversity of Toronto-
Autor(es): dc.creatorBraga, Gabriela Pacheco de Almeida-
Autor(es): dc.creatorCaiaffa, Karina Sampaio-
Autor(es): dc.creatorPereira, Jesse Augusto-
Autor(es): dc.creatorSantos, Vanessa Rodrigues dos-
Autor(es): dc.creatorSouza, Amanda Caselato Andolfatto-
Autor(es): dc.creatorRibeiro, Lucas da Silva-
Autor(es): dc.creatorCamargo, Emerson Rodrigues-
Autor(es): dc.creatorPrakki, Anuradha-
Autor(es): dc.creatorDuque, Cristiane-
Data de aceite: dc.date.accessioned2025-08-21T17:58:34Z-
Data de disponibilização: dc.date.available2025-08-21T17:58:34Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jfb13040305-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248091-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248091-
Descrição: dc.descriptionThis study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)—PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against Enterococcus faecalis, Actinomyces israelii, Lactobacillus casei, Streptococcus mutans, and Fusobacterium nucleatum by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against S. mutans, A. israelli and F. nucleatum. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes.-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemistry Federal University of São Carlos (UFSCar), São Paulo-
Descrição: dc.descriptionDepartment of Clinical Sciences-Restorative Dentistry Faculty of Dentistry University of Toronto-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada: 2018-06489-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada: 2018-06489-
Idioma: dc.languageen-
Relação: dc.relationJournal of Functional Biomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilms-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjectflavonoids-
Palavras-chave: dc.subjecthydrogel-
Título: dc.titleMicrobiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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