New multienzymatic complex formed between human cathepsin D and snake venom phospholipase A2

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Autor(es): dc.contributorOswaldo Cruz Foundation (FIOCRUZ)-
Autor(es): dc.contributorFederal University of Rondônia (UNIR)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorINCT-EPIAMO-
Autor(es): dc.contributorSmart Active Ingredients Lab (SAIL)-
Autor(es): dc.contributorUniversity of Buenos Aires (UBA)-
Autor(es): dc.contributorScience and Technology of Rondonia (IFRO)-
Autor(es): dc.contributorSão Lucas University Center (UniSL)-
Autor(es): dc.contributorAparicio Carvalho University Center (FIMCA)-
Autor(es): dc.creatorMoraes, Jeane do Nascimento-
Autor(es): dc.creatorFrancisco, Aleff Ferreira-
Autor(es): dc.creatorDill, Leandro Moreira-
Autor(es): dc.creatorDiniz, Rafaela Souza-
Autor(es): dc.creatorde Oliveira, Claudia Siqueira-
Autor(es): dc.creatorda Silva, Tainara Maiane Rodrigues-
Autor(es): dc.creatorCaldeira, Cleópatra Alves da Silva-
Autor(es): dc.creatorCorrêa, Edailson de Alcântara-
Autor(es): dc.creatorCoutinho-Neto, Antônio-
Autor(es): dc.creatorZanchi, Fernando Berton-
Autor(es): dc.creatorFontes, Marcos Roberto de Mattos-
Autor(es): dc.creatorSoares, Andreimar Martins-
Autor(es): dc.creatorCalderon, Leonardo de Azevedo-
Data de aceite: dc.date.accessioned2025-08-21T22:12:49Z-
Data de disponibilização: dc.date.available2025-08-21T22:12:49Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1678-9199-JVATITD-2022-0002-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248030-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248030-
Descrição: dc.descriptionBackground: Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis. The overexpression and hypersecretion of CatD have been correlated with cancer aggressiveness and tumor progression, stimulating cancer cell proliferation, fibroblast growth, and angiogenesis. In addition, some studies report its participation in neurodegenerative diseases and inflammatory processes. In this regard, the search for new inhibitors from natural products could be an alternative against the harmful effects of this enzyme. Methods: An investigation was carried out to analyze CatD interaction with snake venom toxins in an attempt to find inhibitory molecules. Interestingly, human CatD shows the ability to bind strongly to snake venom phospholipases A2 (svPLA2), forming a stable muti-enzymatic complex that maintains the catalytic activity of both CatD and PLA2. In addition, this complex remains active even under exposure to the specific inhibitor pepstatin A. Furthermore, the complex formation between CatD and svPLA2 was evidenced by surface plasmon resonance (SPR), two-dimensional electrophoresis, enzymatic assays, and extensive molecular docking and dynamics techniques. Conclusion: The present study suggests the versatility of human CatD and svPLA2, showing that these enzymes can form a fully functional new enzymatic complex.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCenter for the Study of Biomolecules Applied to Health (CEBio) Oswaldo Cruz Foundation (FIOCRUZ), RO-
Descrição: dc.descriptionGraduate Program in Experimental Biology (PGBIOEXP) Department of Medicine (DEPMED) Federal University of Rondônia (UNIR), RO-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Biotechnology of Proteins and Bioactive Compounds (LABIOPROT) Oswaldo Cruz Foundation (FIOCRUZ), RO-
Descrição: dc.descriptionNational Institute of Science and Technology of Epidemiology of the Western Amazon INCT-EPIAMO, RO-
Descrição: dc.descriptionSmart Active Ingredients Lab (SAIL), RO-
Descrição: dc.descriptionFaculty of Medicine University of Buenos Aires (UBA)-
Descrição: dc.descriptionFederal Institute of Education Science and Technology of Rondonia (IFRO), RO-
Descrição: dc.descriptionBioinformatics and Medicinal Chemistry Laboratory (LABIOQUIM) Oswaldo Cruz Foundation (FIOCRUZ), RO-
Descrição: dc.descriptionSão Lucas University Center (UniSL), RO-
Descrição: dc.descriptionAparicio Carvalho University Center (FIMCA), RO-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Venomous Animals and Toxins Including Tropical Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCathepsin D-
Palavras-chave: dc.subjectEnzyme complex-
Palavras-chave: dc.subjectPhospholipases A2-
Palavras-chave: dc.subjectSnake venom-
Título: dc.titleNew multienzymatic complex formed between human cathepsin D and snake venom phospholipase A2-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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