Detoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDias de Oliveira, Luciane-
Autor(es): dc.creatorde Oliveira, Felipe Eduardo-
Autor(es): dc.creatorHatje, Bárbara Araujo-
Autor(es): dc.creatorValera, Marcia Carneiro-
Autor(es): dc.creatorCarvalho, Cláudio Antonio Talge-
Autor(es): dc.creatorHasna, Amjad Abu-
Data de aceite: dc.date.accessioned2025-08-21T20:02:08Z-
Data de disponibilização: dc.date.available2025-08-21T20:02:08Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/0103-6440202205195-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/248002-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/248002-
Descrição: dc.descriptionThe aim of this study was to evaluate in vitro the effect of calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) on macrophages (RAW 264.7) to produce pro-inflammatory cytokines and nitric oxide after pretreatment with lipoteichoic acid (LTA) of Enterococcus faecalis. Forty-eight human single- rooted teeth were instrumented with R25.08 (RECIPROC) and sterilized by gamma irradiation. LTA was inoculated in the root canal of each specimen for 96 hours. Specimens were instrumented with 40.06 and 50.05 (RECIPROC) and medicated with: I) Pyrogen-free saline solution (SS); II) 2% CHX gel; III) Ca(OH)2 + SS; or IV) Ca(OH)2 + CHX for 14 days. Three samples (S) were performed of the root canal of each specimen at: S1) immediately after instrumentation; S2) after Ethylenediaminetetraacetic acid (EDTA); S3) after intracanal medication removal. Subsequent quantification of cytokines (IL-1 β, TNF-α, MIP-1α, IP-10, G-CSF and IL-6) by immunosorbent assay (ELISA) and nitric oxide by the Griess method was carried-out. Data were submitted to a normality test and then analyzed with one-way ANOVA and Tukey test with a significance level of 5% using GraphPad Prism 6. Ca(OH)2 + SS and Ca(OH)2 + CHX presented lower levels of TNF-α, TNF-α, IL-6, G-CSF and nitric oxide. Ca(OH)2 + SS was the most effective in reducing MIP-1α. CHX was effective in reducing IL-6 and G-CSF. Therefore, the combined intracanal medication of calcium hydroxide and chlorhexidine is effective in reducing the cytokines TNF-α, IL-1β, IL-6, G-CSF and nitric oxide.-
Descrição: dc.descriptionDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos Campos-
Descrição: dc.descriptionDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos Campos-
Descrição: dc.descriptionDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESP, São José dos Campos-
Descrição: dc.descriptionDepartment of Restorative Dentistry Endodontics division Institute of Science and Technology Saõ Paulo State University - UNESP, Saõ Jose dos Campos-
Formato: dc.format36-43-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Dental Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcalcium hydroxide-
Palavras-chave: dc.subjectchlorhexidine-
Palavras-chave: dc.subjectcytokines-
Palavras-chave: dc.subjectlipoteichoic acid-
Palavras-chave: dc.subjectmacrophages-
Título: dc.titleDetoxification of LTA by intracanal medication: analysis by macrophages proinflammatory cytokines production-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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