Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorReproductive Medicine Centre-
Autor(es): dc.contributorUniversity of Milan-
Autor(es): dc.creatorDellaqua, Thaisy Tino-
Autor(es): dc.creatorVígaro, Renan Aparecido-
Autor(es): dc.creatorJanini, Ludimila Cardoso Zoccal-
Autor(es): dc.creatorDal Canto, Mariabeatrice-
Autor(es): dc.creatorRenzini, Mario Mignini-
Autor(es): dc.creatorLodde, Valentina-
Autor(es): dc.creatorLuciano, Alberto Maria-
Autor(es): dc.creatorBuratini, Jose-
Data de aceite: dc.date.accessioned2025-08-21T16:05:29Z-
Data de disponibilização: dc.date.available2025-08-21T16:05:29Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2022.10.041-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247930-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247930-
Descrição: dc.descriptionOocyte in vitro maturation (IVM) is still a major challenge in human and animal assisted reproduction. Gradual instead of abrupt activation of the ovulatory cascade during IVM has been proposed to enhance nuclear-cytoplasmic synchrony and cumulus-oocyte communication, thus favoring oocyte developmental competence. Herein, we assessed the effects of neuregulin 1 (NRG1), an EGF-like factor that modulates EGFR signaling, on oocyte nuclear maturation dynamics, cumulus expansion and expression of mRNAs regulating these processes during IVM, as well as on post-IVF embryo development following AREG-stimulated IVM in cattle. In experiment 1, cumulus-oocyte complexes (COCs) were subjected to IVM with graded doses of NRG1 (1, 10 or 100 ng/mL) for 6, 9, 12, 20, and 24 h, after which oocyte nuclear status and cumulus mRNA expression were assessed. At 6 h of IVM, NRG1 at 1 ng/mL significantly decreased the percentage of GVBD (germinal vesicle breakdown) oocytes without altering later meiotic dynamics or the percentage of oocytes achieving meiosis II. In experiment 2, adding NRG1 (1 ng/mL) to the IVM medium did not affect cumulus expansion but increased the percentage of expanded and hatched blastocysts, and blastocyst total cell number following IVF/IVC. NRG1 decreased EGFR mRNA abundance while increasing NPR2 and PTX3 mRNA levels at 9 h, and TNFAIP6 mRNA abundance at 20 h of IVM. This is the first study that reports the modulatory effect of NGR1 during oocyte maturation in a mono-ovulatory species and demonstrates that this action may be applied during IVM to improve post-IVF embryo development.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State University, Sao Paulo-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Sao Paulo State University, Sao Paulo-
Descrição: dc.descriptionBiogenesi Reproductive Medicine Centre-
Descrição: dc.descriptionReproductive and Developmental Biology Laboratory Department of Veterinary Medicine and Animal Science University of Milan-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State University, Sao Paulo-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Sao Paulo State University, Sao Paulo-
Descrição: dc.descriptionCAPES: 0001-
Descrição: dc.descriptionFAPESP: 2019/14588–6-
Formato: dc.format209-216-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAREG-
Palavras-chave: dc.subjectBovine embryo production-
Palavras-chave: dc.subjectEGF-Like-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectOocyte nuclear maturation-
Título: dc.titleNeuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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