Combined physical exercise reverses the reduced expression of Bmal1 in the liver of aged mice

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorTufts University-
Autor(es): dc.creatorPinto, Ana P.-
Autor(es): dc.creatorMuñoz, Vitor R.-
Autor(es): dc.creatorTavares, Maria Eduarda A.-
Autor(es): dc.creatordos Santos, Jonathas R.-
Autor(es): dc.creatorRebelo, Macario A.-
Autor(es): dc.creatorAlberici, Luciane C.-
Autor(es): dc.creatorSimabuco, Fernando M.-
Autor(es): dc.creatorTeixeira, Giovana R.-
Autor(es): dc.creatorPauli, José R.-
Autor(es): dc.creatorde Moura, Leandro P.-
Autor(es): dc.creatorCintra, Dennys E.-
Autor(es): dc.creatorRopelle, Eduardo R.-
Autor(es): dc.creatorFreitas, Ellen C.-
Autor(es): dc.creatorRivas, Donato A.-
Autor(es): dc.creatorda Silva, Adelino S.R.-
Data de aceite: dc.date.accessioned2025-08-21T21:14:34Z-
Data de disponibilização: dc.date.available2025-08-21T21:14:34Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2022.121175-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247926-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247926-
Descrição: dc.descriptionAging can modify the morphology and function of the liver, such as generating a decrease in the mitochondria content, autophagy, and cell senescence. Although exercise training has several beneficial effects on hepatic metabolism, its actions on autophagy processes, mitochondrial function, and cellular senescence need to be more widely explored. The present study verified the effects of aging and exercise on hepatic circadian markers, autophagy, and mitochondria activity in 24-month-old mice with a combined exercise training protocol. In addition, we used public datasets from human livers in several conditions and BMAL1 knockout mice. C57BL/6 mice were distributed into Control (CT, young, 6-month-old mice), sedentary old (Old Sed, sedentary, 24-month-old mice), and exercised old (Old Ex, 24-month-old mice submitted to a combined exercise training protocol). The exercise training protocol consisted of three days of endurance exercise – treadmill running, and two days of resistance exercise – climbing a ladder, for three weeks. At the end of the protocol, the liver was removed and prepared for histological analysis, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), immunoblotting technique, and oxygen consumption. Heatmaps were built using a human dataset and Bmal1 knockout samples. In summary, the Old Sed had reduced strength, coordination, and balance, as well as a decrease in Bmal1 expression and the presence of degenerated liver cells. Still, this group upregulated the transcription factors related to mitochondrial biogenesis. The Old Ex group had increased strength, coordination, and balance, improved glucose sensitivity, as well as restored Bmal1 expression and the mitochondrial transcription factors. The human datasets indicated that mitochondrial markers and autophagy strongly correlate with specific liver diseases but not aging. We can speculate that mitochondrial and autophagy molecular markers alterations may depend on long-term training.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSchool of Physical Education and Sport of Ribeirão Preto University of São Paulo (USP), São Paulo-
Descrição: dc.descriptionMulticentric Program of Postgraduate in Physiological Sciences São Paulo State University (UNESP) School of Dentistry of Araçatuba, São Paulo-
Descrição: dc.descriptionDepartment of Biomolecular Sciences School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo-FCFRP USP, Sao Paulo-
Descrição: dc.descriptionDepartment of Pharmacology Faculty of Medicinal Sciences University of Campinas State University of Campinas (UNICAMP), São Paulo-
Descrição: dc.descriptionLaboratory of Molecular Biology of Exercise (LaBMEx) School of Applied Sciences University of Campinas (UNICAMP), São Paulo-
Descrição: dc.descriptionDepartment of Physical Education State University of São Paulo (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Health Sciences Medical School University of Sao Paulo, Sao Paulo-
Descrição: dc.descriptionNutrition Exercise Physiology and Sarcopenia Laboratory Tufts University-
Descrição: dc.descriptionPostgraduate Program in Rehabilitation and Functional Performance Ribeirão Preto Medical School University of São Paulo (USP), São Paulo-
Descrição: dc.descriptionDepartment of Biochemistry Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionMulticentric Program of Postgraduate in Physiological Sciences São Paulo State University (UNESP) School of Dentistry of Araçatuba, São Paulo-
Descrição: dc.descriptionDepartment of Physical Education State University of São Paulo (UNESP), São Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2019/11820-5-
Descrição: dc.descriptionFAPESP: 2020/13443-1-
Descrição: dc.descriptionFAPESP: 2021/06291-3-
Descrição: dc.descriptionFAPESP: 2021/08692-5-
Descrição: dc.descriptionFAPESP: 2021/08693-1-
Descrição: dc.descriptionCNPq: 301279/2019-5-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCombined exercise-
Palavras-chave: dc.subjectLiver-
Palavras-chave: dc.subjectRev-erbα-
Palavras-chave: dc.subjectSenescence-
Palavras-chave: dc.subjectTranscriptome-
Título: dc.titleCombined physical exercise reverses the reduced expression of Bmal1 in the liver of aged mice-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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