Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorState University of Maringa-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Illinois at Chicago-
Autor(es): dc.creatorPiovesana, Tadeu Ravazi-
Autor(es): dc.creatorRodrigues, Lenize da Silva-
Autor(es): dc.creatorBovolato, Ana Livia de Carvalho-
Autor(es): dc.creatorRodríguez-Sánchez, Diego Noé-
Autor(es): dc.creatorRinaldi, Jaqueline Carvalho-
Autor(es): dc.creatorSantos, Nilton José-
Autor(es): dc.creatorMori, Julia Calvi-
Autor(es): dc.creatorLourenção, Pedro Luiz Toledo de Arruda-
Autor(es): dc.creatorBirch, Lynn-
Autor(es): dc.creatorBertanha, Matheus-
Data de aceite: dc.date.accessioned2025-08-21T20:34:25Z-
Data de disponibilização: dc.date.available2025-08-21T20:34:25Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biomedicines10112814-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247880-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247880-
Descrição: dc.descriptionBackground: To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces. Methods: New Zealand rabbits (n = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm2) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm2) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scanning electron microscopy (SEM). Results: The integrity of the urinary bladder was maintained in both groups. A superior regenerative process was observed in the G2 group, compared to the G1 group. We observed a greater urothelial epithelialization and maturity of the mucosa and submucosa fibroblasts. Furthermore, SEM demonstrated a notable amount of urothelial villus in the G2 group. Conclusion: Decellularized vena cava scaffolds were able to maintain the integrity of the urinary bladder in the proposed model. In addition, ASCs accelerated the regenerative process development, observed primarily by the new urothelial epithelization and the maturity of mucosa and submucosa fibroblasts.-
Descrição: dc.descriptionDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University-UNESP-
Descrição: dc.descriptionApplied Biotechnology Laboratory Clinical Hospital of Botucatu Medical School São Paulo State University-UNESP-
Descrição: dc.descriptionPostgraduate Program in Biosciences and Physiopathology State University of Maringa-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience of Botucatu São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology University of Campinas–UNICAMP-
Descrição: dc.descriptionDepartment of Urology College of Medicine University of Illinois at Chicago-
Descrição: dc.descriptionDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University-UNESP-
Descrição: dc.descriptionApplied Biotechnology Laboratory Clinical Hospital of Botucatu Medical School São Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience of Botucatu São Paulo State University—UNESP-
Idioma: dc.languageen-
Relação: dc.relationBiomedicines-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectmesenchymal stem cell-
Palavras-chave: dc.subjecttissue engineering-
Palavras-chave: dc.subjecturinary bladder-
Palavras-chave: dc.subjecturinary bladder diseases-
Palavras-chave: dc.subjecturologic surgical procedures-
Título: dc.titleUrinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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