Long-Term Wastewater Surveillance for SARS-CoV-2: One-Year Study in Brazil

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity Feevale-
Autor(es): dc.contributorSeMAE-Autonomous Municipal Water and Sewage Service-
Autor(es): dc.contributorFaculty of Medicine of São José do Rio Preto (FAMERP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorMartins, Renan Moura-
Autor(es): dc.creatorCarvalho, Tamara-
Autor(es): dc.creatorBittar, Cintia-
Autor(es): dc.creatorQuevedo, Daniela Muller-
Autor(es): dc.creatorMiceli, Rafael Nava-
Autor(es): dc.creatorNogueira, Mauricio Lacerda-
Autor(es): dc.creatorFerreira, Helena Lage-
Autor(es): dc.creatorCosta, Paulo Inácio-
Autor(es): dc.creatorAraújo, João Pessoa-
Autor(es): dc.creatorSpilki, Fernando Rosado-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorCalmon, Marilia Freitas-
Data de aceite: dc.date.accessioned2025-08-21T19:03:22Z-
Data de disponibilização: dc.date.available2025-08-21T19:03:22Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/v14112333-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247862-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247862-
Descrição: dc.descriptionWastewater-based epidemiology (WBE) is a tool involving the analysis of wastewater for chemicals and pathogens at the community level. WBE has been shown to be an effective surveillance system for SARS-CoV-2, providing an early-warning-detection system for disease prevalence in the community via the detection of genetic materials in the wastewater. In numerous nation-states, studies have indicated the presence of SARS-CoV-2 in wastewater. Herein, we report the primary time-course monitoring of SARS-CoV-2 RNA in wastewater samples in São José do Rio Preto-SP/Brazil in order to explain the dynamics of the presence of SARS-CoV-2 RNA during one year of the SARS-CoV-2 pandemic and analyze possible relationships with other environmental parameters. We performed RNA quantification of SARS-CoV-2 by RT-qPCR using N1 and N2 targets. The proportion of positive samples for every target resulted in 100% and 96.6% for N1 and N2, respectively. A mean lag of -5 days is observed between the wastewater signal and the new SARS-CoV-2-positive cases reported. A correlation was found between the air and wastewater temperatures and therefore between the SARS-CoV-2 viral titers for N1 and N2 targets. We also observed a correlation between SARS-CoV-2 viral titers and media wastewater flow for the N1 target. In addition, we observed higher viral genome copies within the wastewater samples collected on non-rainy days for the N1 target. Thus, we propose that, based on our results, monitoring raw wastewater may be a broadly applicable strategy that might contribute to resolving the pressing problem of insufficient diagnostic testing; it may represent an inexpensive and early-warning method for future COVID-19 outbreaks, mainly in lower- and middle-income countries.-
Descrição: dc.descriptionLaboratory of Genomic Studies Institute of Biosciences Letters and Exact Sciences (IBILCE) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInstitute of Exact and Technological Sciences (ICET) University Feevale, RS-
Descrição: dc.descriptionSeMAE-Autonomous Municipal Water and Sewage Service, SP-
Descrição: dc.descriptionVirology Research Laboratory (LPV) Faculty of Medicine of São José do Rio Preto (FAMERP), SP-
Descrição: dc.descriptionApplied Preventive Veterinary Medicine Laboratory Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of São Paulo (USP), SP-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionBiotechnology Institute São Paulo State University (UNESP), SP-
Descrição: dc.descriptionMolecular Microbiology Laboratory University Feevale, RS-
Descrição: dc.descriptionLaboratory of Genomic Studies Institute of Biosciences Letters and Exact Sciences (IBILCE) São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionBiotechnology Institute São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationViruses-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectepidemiology-
Palavras-chave: dc.subjectqRT-PCR-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectwastewater-
Título: dc.titleLong-Term Wastewater Surveillance for SARS-CoV-2: One-Year Study in Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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