The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSchool of Dentistry-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCassiano, Ana Flávia Balestrero-
Autor(es): dc.creatorCoaguila-Llerena, Hernán-
Autor(es): dc.creatorSantos, Cíntia Silva-
Autor(es): dc.creatorda Silva, Luana Raphael-
Autor(es): dc.creatorNogueira, Lucas Fabrício Bahia-
Autor(es): dc.creatorCiancaglini, Pietro-
Autor(es): dc.creatorFaria, Gisele-
Data de aceite: dc.date.accessioned2025-08-21T21:17:44Z-
Data de disponibilização: dc.date.available2025-08-21T21:17:44Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.joen.2022.09.010-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247837-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247837-
Descrição: dc.descriptionIntroduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SP-
Descrição: dc.descriptionDivision of Endodontics University of Minnesota School of Dentistry-
Descrição: dc.descriptionDepartment of Chemistry Ribeirão Preto College of Philosophy Sciences and Letters São Paulo University – USP, SP-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University -UNESP, SP-
Descrição: dc.descriptionFAPESP: 18/24662-6-
Descrição: dc.descriptionFAPESP: 20/09576-6-
Formato: dc.format1502-1510.e1-
Idioma: dc.languageen-
Relação: dc.relationJournal of Endodontics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApical papilla stem cells-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjectdental pulp stem cells-
Palavras-chave: dc.subjectoctenidine-
Palavras-chave: dc.subjectregenerative endodontics-
Título: dc.titleThe Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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