A SARS-CoV-2 impedimetric biosensor based on the immobilization of ACE-2 receptor-containing entire cell membranes as the biorecognition element

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCancino-Bernardi, Juliana-
Autor(es): dc.creatorComparetti, Edson José-
Autor(es): dc.creatorFerreira, Natalia Noronha-
Autor(es): dc.creatorMiranda, Renata Rank-
Autor(es): dc.creatorTuesta, Marco Montero-
Autor(es): dc.creatorSampaio, Isabella-
Autor(es): dc.creatorInácio da Costa, Paulo-
Autor(es): dc.creatorZucolotto, Valtencir-
Data de aceite: dc.date.accessioned2025-08-21T21:46:57Z-
Data de disponibilização: dc.date.available2025-08-21T21:46:57Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2022.124008-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247806-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247806-
Descrição: dc.descriptionA SARS-CoV-2 biosensor based on the biorecognition of the spike protein to the angiotensin-converting enzyme 2 (ACE-2) transmembrane receptor was developed using entire cell membranes as the biorecognition layer. In this new SARS-CoV-2 detection platform, cellular membranes from VeroCCL81 (mVero) and Calu-3 (mCalu) cells (which overexpress the ACE-2 transmembrane receptors) were extracted and immobilized as vesicles on an indium tin oxide electrode (ITO). Electrochemical impedance spectroscopy was used to optimize the performance of the developed devices for SARS-CoV-2 detection. This novel biosensor comprises a low-cost system (less than one US$ dollar) that uses the unique properties of cell membranes combined with the catalytic properties of electrochemical platforms to allow spike proteins recognition. A linear response from 10 to 100 ng/mL was obtained from the optimized biosensors, a limit of detection of 10.0 pg/mL and 7.25 pg/mL and limit of quantification of 30.4 pg/mL and 21.9 pg/mL were achieved with satisfactory accuracy for ITO-APTES-mVero and ITO-APTES-mCalu, respectively. Selectivity studies revealed that this platform was able to differentiate the target spike proteins from NS1 proteins from dengue and Zika viruses. In addition, sensors comprising cell membranes devoid of the ACE-2 transmembrane receptor exhibited no biorecognition signal. The developed devices are suitable for SARS-CoV-2 detection based on spike protein recognition, and capable of providing a low-cost, accurate, and accessible tool for use in a pandemic and post-pandemic scenario.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionNanomedicine and Nanotoxicology Group Physics Institute of São Carlos University of São Paulo, SP-
Descrição: dc.descriptionBioanalytics of Nanosystems Laboratory Department of Chemistry FFCLRP-USP University of São Paulo – USP, SP-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University Department of Clinical Analysis Laboratory of Clinical Immunology and Molecular Biology, São Paulo-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo State University Department of Clinical Analysis Laboratory of Clinical Immunology and Molecular Biology, São Paulo-
Idioma: dc.languageen-
Relação: dc.relationTalanta-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectACE-2 transmembrane receptor-
Palavras-chave: dc.subjectBiosensor-
Palavras-chave: dc.subjectCovid-19-
Palavras-chave: dc.subjectSARS-CoV-2-
Título: dc.titleA SARS-CoV-2 impedimetric biosensor based on the immobilization of ACE-2 receptor-containing entire cell membranes as the biorecognition element-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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