Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMarilia University (UNIMAR)-
Autor(es): dc.contributorUniversidad CES-
Autor(es): dc.contributorUniversity of Naples “Federico II”-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorNogueira Aires, Luiz Paulo-
Autor(es): dc.creatorGasser, Beatriz-
Autor(es): dc.creatorSilva, Priscila-
Autor(es): dc.creatorDel'Aguila-Silva, Priscila-
Autor(es): dc.creatorYamada, Diego Iwao-
Autor(es): dc.creatorCarneiro, Rafael Kretzer-
Autor(es): dc.creatorBruna Bressianini Lima, Bruna-
Autor(es): dc.creatorPadilha-Nakaghi, Luciana Cristina-
Autor(es): dc.creatorRamirez Uscategui, Ricardo Andrés-
Autor(es): dc.creatorSpada, Stefano-
Autor(es): dc.creatorRusso, Marco-
Autor(es): dc.creatorRossi Feliciano, Marcus Antônio-
Data de aceite: dc.date.accessioned2025-08-21T16:49:18Z-
Data de disponibilização: dc.date.available2025-08-21T16:49:18Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2022.10.009-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247738-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247738-
Descrição: dc.descriptionThe aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast-Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 – T5) of the estrous cycle established by vaginal cytology and serum progesterone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color-coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue®, Bracco, Sao Paulo, Brazil) and the CADENCE™ Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demonstrated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao Paulo-
Descrição: dc.descriptionMarilia University (UNIMAR), Sao Paulo-
Descrição: dc.descriptionResearch Group INCA-CES Faculty of Veterinary Medicine and Animal Science Universidad CES-
Descrição: dc.descriptionDepartment of Veterinary Medicine and Animal Productions University of Naples “Federico II”-
Descrição: dc.descriptionLaboratory of Veterinary Imaginology Faculty of Animal Science and Food Engineering (FZEA) Sao Paulo University (USP), Sao Paulo-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao Paulo-
Descrição: dc.descriptionDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao Paulo-
Descrição: dc.descriptionCNPq: nº 130820/2019-9-
Descrição: dc.descriptionFAPESP: nº 2017/14957-6-
Descrição: dc.descriptionFAPESP: nº 2019/15282-8-
Descrição: dc.descriptionCNPq: nº 309199/2017-4-
Descrição: dc.descriptionCNPq: nº 430023/2018-9-
Formato: dc.format162-170-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCanine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesis-
Título: dc.titleOvarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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