Identification, cloning, and characterization of a novel chitinase from leaf-cutting ant Atta sexdens: An enzyme with antifungal and insecticidal activity

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorMicocci, Kelli C.-
Autor(es): dc.creatorMoreira, Ariele C.-
Autor(es): dc.creatorSanchez, Amanda D.-
Autor(es): dc.creatorPettinatti, Jessica L.-
Autor(es): dc.creatorRocha, Marina C.-
Autor(es): dc.creatorDionizio, Bruna S.-
Autor(es): dc.creatorCorrea, Katia C.S.-
Autor(es): dc.creatorMalavazi, Iran-
Autor(es): dc.creatorWouters, Felipe C.-
Autor(es): dc.creatorBueno, Odair C.-
Autor(es): dc.creatorSouza, Dulce Helena F.-
Data de aceite: dc.date.accessioned2025-08-21T18:33:48Z-
Data de disponibilização: dc.date.available2025-08-21T18:33:48Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbagen.2022.130249-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247732-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247732-
Descrição: dc.descriptionChitinases are enzymes that degrade chitin, a polysaccharide found in the exoskeleton of insects, fungi, yeast, and internal structures of other vertebrates. Although chitinases isolated from bacteria, fungi and plants have been reported to have antifungal or insecticide activities, chitinases from insects with these activities have been seldomly reported. In this study, a leaf-cutting ant Atta sexdens DNA fragment containing 1623 base pairs was amplified and cloned into a vector to express the protein (AsChtII-C4B1) in Pichia pastoris. AsChtII-C4B1, which contains one catalytic domain and one carbohydrate-binding module (CBM), was secreted to the extracellular medium and purified by ammonium sulfate precipitation followed by nickel column chromatography. AsChtII-C4B1 showed maximum activity at pH 5.0 and 55 °C when tested against colloidal chitin substrate and maintained >60% of its maximal activity in different temperatures during 48 h. AsChtII-C4B1 decreased the survival of Spodoptera frugiperda larvae fed with an artificial diet that contained AsChtII-C4B1. Our results have indicated that AsChtII-C4B1 has a higher effect on larva-pupa than larva-larva molts. AsChtII-C4B1 activity targets more specifically the growth of filamentous fungus than yeast. This work describes, for the first time, the obtaining a recombinant chitinase from ants and the characterization of its insecticidal and antifungal activities.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCenter for the Study of Social Insects São Paulo State University “Julio de Mesquita Filho”, Rio Claro, SP-
Descrição: dc.descriptionDepartment of Physics Chemistry and Mathematics Federal University of São Carlos, SP-
Descrição: dc.descriptionDepartment of Chemistry Federal University of São Carlos, SP-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos, SP-
Descrição: dc.descriptionCenter for the Study of Social Insects São Paulo State University “Julio de Mesquita Filho”, Rio Claro, SP-
Descrição: dc.descriptionFAPESP: 2017/06198-8-
Idioma: dc.languageen-
Relação: dc.relationBiochimica et Biophysica Acta - General Subjects-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntifungal activity-
Palavras-chave: dc.subjectChitinase insect-
Palavras-chave: dc.subjectInsecticide activity-
Título: dc.titleIdentification, cloning, and characterization of a novel chitinase from leaf-cutting ant Atta sexdens: An enzyme with antifungal and insecticidal activity-
Tipo de arquivo: dc.typelivro digital-
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