Comparison of the Physicochemical Properties, Antimicrobial Action, and Cytotoxicity of Ambroxol Hydrochloride, N-acetylcysteine, and Calcium Hydroxide Pastes

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity Sagrado Coração-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUnimed Hospital-
Autor(es): dc.creatorSouza Calefi, Pedro Henrique-
Autor(es): dc.creatorde Azevedo Queiroz, India Olinta-
Autor(es): dc.creatorAlcalde, Murilo Priori-
Autor(es): dc.creatorde Oliveira, Sandra Helena Penha-
Autor(es): dc.creatorVivan, Rodrigo Ricci-
Autor(es): dc.creatorWeckwerth, Paulo Henrique-
Autor(es): dc.creatorKato, Augusto Shoji-
Autor(es): dc.creatorDuarte, Marco Antonio Hungaro-
Data de aceite: dc.date.accessioned2025-08-21T23:47:46Z-
Data de disponibilização: dc.date.available2025-08-21T23:47:46Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.14744/eej.2022.30306-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247722-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247722-
Descrição: dc.descriptionObjective: To evaluate the solubility, pH, antimicrobial action, and cytotoxicity of ambroxol hydrochloride (AMB), N-acetylcysteine (NAC), and calcium hydroxide (CH) pastes for use as intracanal medications. Methods: Solubility was determined by micro-CT, based on the paste volume remaining after immersion in water for 7 days. pH was measured by immersing acrylic tubes containing the pastes in ultrapure water and then measuring pH after 3 hours, 3 days, and 7 days. Antimicrobial action against Enterococcus faecalis was assessed based on the percentage of living cells, using the live/dead staining method under confocal microscopy. Cytotoxicity was assessed based on the cell viability of L929 fibroblast-like cells after 6, 24, and 48 hours. Cytotoxicity data were compared using the ANOVA and Tukey tests, and the antimicrobial data were compared using the Kruskal-Wallis and Dunn tests. The significance level used was 5% (α=0.05). Results: The solubility values for all the study groups were significantly different (P<0.05), where the highest values were for NAC, followed by AMB, and then CH. Likewise, the pH levels were all significantly different (P<0.05), where NAC and AMB levels were acidic, and CH levels were alkaline. The antimicrobial action of AMB was significantly higher than that of CH (P<0.05), and that of NAC was also higher than that of CH, albeit not significantly. AMB and NAC were more cytotoxic than CH, and higher dilutions of CH promoted higher cell viability levels than lower dilutions of the same paste (P<0.05). Conclusion: : The NAC and AMB pastes were more soluble and cytotoxic than the CH paste and had acidic pH levels. The AMB paste displayed the highest antimicrobial action against Enterococcus faecalis biofilm.-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Dentistry University Sagrado Coração, Bauru-
Descrição: dc.descriptionDepartment of Basic Science Araçatuba Dental School UNESP, Araçatuba-
Descrição: dc.descriptionDepartment of Mibrobiology Unimed Hospital, Bauru-
Descrição: dc.descriptionDepartment of Basic Science Araçatuba Dental School UNESP, Araçatuba-
Formato: dc.format217-222-
Idioma: dc.languageen-
Relação: dc.relationEuropean Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcetylcysteine-
Palavras-chave: dc.subjectambroxol hydrochloride-
Palavras-chave: dc.subjectcalcium hydroxide-
Palavras-chave: dc.subjectEnterococcus faecalis-
Palavras-chave: dc.subjectintracanal medicaments-
Título: dc.titleComparison of the Physicochemical Properties, Antimicrobial Action, and Cytotoxicity of Ambroxol Hydrochloride, N-acetylcysteine, and Calcium Hydroxide Pastes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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