Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorThe University of British Columbia-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorLeite, Maria Luísa-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorSoares, Igor Paulino Mendes-
Autor(es): dc.creatorManso, Adriana Pigozzo-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorCarvalho, Ricardo Marins-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Data de aceite: dc.date.accessioned2025-08-21T23:38:05Z-
Data de disponibilização: dc.date.available2025-08-21T23:38:05Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2022.09.014-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247711-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247711-
Descrição: dc.descriptionObjectives: This study aimed to develop and characterize different formulations of porous chitosan scaffolds (SCH) associated with calcium silicate (CaSi) and evaluate their chemotactic and bioactive potential on human dental pulp cells (hDPCs). Methods: Different concentrations of CaSi suspensions (0.5%, 1.0%, and 2.0%, w/v) were incorporated (1:5; v/v) /or not, into 2% chitosan solution, giving rise to the following groups: SCH (control); SCH+ 0.5CaSi; SCH+ 1.0CaSi; SCH+ 2.0 CaSi. The resulting solutions were submitted to thermally induced phase separation followed by freeze-drying procedures to obtain porous scaffolds. The topography, pH, and calcium release kinetics of the scaffolds were assessed. Next, the study evaluated the influence of these scaffolds on cell migration (MG), viability (VB), proliferation (PL), adhesion and spreading (A&S), and on total protein synthesis (TP), alkaline phosphatase (ALP) activity, mineralized matrix deposition (MMD), and gene expression (GE) of odontogenic differentiation markers (ALP, DSPP, and DMP-1). The data were analyzed with ANOVA complemented with the Tukey post-hoc test (α = 5%). Results: Incorporation of the CaSi suspension into the chitosan scaffold formulation increased pore diameter when compared with control. Increased amounts of CaSi in the CH scaffold resulted in higher pH values and Ca release. In Groups SCH+ 1.0CaSi and SCH+ 2.0CaSi, increased VB, PF, A&S, GE of DSPP/DMP-1 and MMD values were shown. However, Group SCH+ 2.0CaSi was the only formulation capable of enhancing MG and showed the highest increase in TP, MMD, and GE of DMP-1 and DSPP values. Significance: SCH+ 2.0CaSi formulation had the highest chemotactic and bioactive potential on hDPCs and may be considered a potential biomaterial for pulp-dentin complex regeneration.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Oral Health Sciences Faculty of Dentistry The University of British Columbia, 2199 Wesbrook Mall-
Descrição: dc.descriptionDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionDepartment of Oral Biological & Medical Sciences Faculty of Dentistry The University of British Columbia, 2199 Wesbrook Mall-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry Universidade Estadual Paulista – UNESP, 1680 Humaitá Street-
Descrição: dc.descriptionFAPESP: 2019/14965-4-
Descrição: dc.descriptionFAPESP: 2020/09095-8-
Formato: dc.format1763-1776-
Idioma: dc.languageen-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCalcium silicate-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectDirect pulp capping-
Palavras-chave: dc.subjectPorous scaffold-
Palavras-chave: dc.subjectPulp-dentin complex regeneration-
Palavras-chave: dc.subjectTissue engineering-
Título: dc.titleCalcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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